Active Recombinant Human ECM1, His-tagged

• Cat.No.: ECM1-315H
• Product Overview: Recombinant Human ECM1 (Accession # AAH23505) Ala20-Glu540, fused with a C-terminal 6-His tag, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Species: Human
• Source: Mammalian Cells
• Tag: His
• Protein Length: 20-540 a.a.
• Predicted N Terminal: Ala20
• Form: Lyophilized from a 0.2 µm filtered solution in PBS.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of B16-F1 mouse melanoma cells.When 5 x 10^4 cells/well are added to rhECM-1/His coated plates (10 µg/mL with 100 µL/well), approximately 30 - 65% will adhere after 30 minutes at 37° C. Optimal concentration depends on cell type as well as the application or research objective.
• Molecular Mass: Recombinant Human ECM1, His-tagged has a calculated MW of 59.7 kDa. In SDS-PAGE migrates as 77-87 kDa, reducing conditions.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: ECM1 extracellular matrix protein 1 [ Homo sapiens ]
• Official Symbol: ECM1
• Synonyms: ECM1; extracellular matrix protein 1; secretory component p85
• Gene ID: 1893
• mRNA Refseq: NM_001202858
• Protein Refseq: NP_001189787
• MIM: 602201
• UniProt ID: Q16610
• Chromosome Location: 1q21