Active Recombinant Human EGFL6 protein, His-FLAG-tagged

• Cat.No.: EGFL6-447H
• Product Overview: Active Recombinant Human EGFL6 protein(NP_056322.2) (Asn22-Asp553) was expressed in Insect Cells with the flag tag at the N-terminus and the histidine tag at the C-terminus.

Specification

• Species: Human
• Source: Insect Cells
• Tag: Flag&His
• Protein Length: 22-553 aa
• Form: Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 20 % glycerol, pH 7.4.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of NIH-3T3 mouse embryonic fibroblast cells.When 5 × 10E4 cells/well areadded to CD4 coated plates (1.25μg/mL and 100μL/well),apximately 40%-60% will adhere specifically after 30 minutes at 37°C.
• Molecular Mass: The recombinant human EGFL6 consists of 551 amino acids and has a calculated molecular mass of 61.5 kDa.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 65 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.