Active Recombinant Human ENPP2, His-tagged

• Cat.No.: ENPP2-3123H
• Product Overview: Recombinant human ENPP2/ATX (ectonucleotide pyrophosphatase-phosphodiesterase 2) is a single polypeptide chain with a 8His tag at the C-terminus. It contains 925 (915+10) amino acids, and having a predicted molecular mass of approximately 106.4kD and migrates in SDS-PAGE with an apparent molecular mass of 110kD.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Description: Autotaxin also known as ectonucleotide pyrophosphatase/phosphodiesterase family member 2 (E-NPP 2) is an enzyme that in humans is encoded by the ENPP2 gene.
• Form: 20mM Tris-Cl (pH7.9), 20% Glycerol, 100mM NaCl, 1mM DTT and 0.5mM EDTA
• Bio-activity: ENPP2 protein stimulates the motility of tumor cells with angiogenic properties. It functions as a phosphodiesterase to cleave phosphodiester bonds and a phospholipase to catalyze production of lysophosphatidic acid (LPA). Recombinant human ENPP2 protein is ideal for the studies of its enzyme activity and other related function assays
• AA Sequence: MARRSSFQSCQIISLFTFAVGVNICLGFTAHRIKRAEGWEEGPPTVLSDSPWTNISGSCKGRCFELQEAGPPDC RCDNLCKSYTSCCHDFDELCLKTARGWECTKDRCGEVRNEENACHCSEDCLARGDCCTNYQVVCKGESHWVDDD CEEIKAAECPAGFVRPPLIIFSVDGFRASYMKKGSKVMPNIEKLRSCGTHSPYMRPVYPTKTFPNLYTLATGL YPESHGIVGNSMYDPVFDATFHLRGREKFNHRWWGGQPLWITATKQGVKAGTFFWSVVIPHERRILTILQWLTL PDHERPSVYAFYSEQPDFSGHKYGPFGPEESSYGSPFTPAKRPKRKVAPKRRQERPVAPPKKRRRKIHRMDHY AAETRQDKMTNPLREIDKIVGQLMDGLKQLKLHRCVNVIFVGDHGMEDVTCDRTEFLSNYLTNVDDITLVPGTL GRIRSKFSNNAKYDPKAIIANLTCKKPDQHFKPYLKQHLPKRLHYANNRRIEDIHLLVERRWHVARKPLDVYK KPSGKCFFQGDHGFDNKVNSMQTVFVGYGSTFKYKTKVPPFENIELYNVMCDLLGLKPAPNNGTHGSLNHLLRT NTFRPTMPEEVTRPNYPGIMYLQSDFDLGCTCDDKVEPKNKLDELNKRLHTKGSTEERHLLYGRPAVLYRTRY DILYHTDFESGYSEIFLMPLWTSYTVSKQAEVSSVPDHLTSCVRPDVRVSPSFSQNCLAYKNDKQMSYGFLFPP YLSSSPEAKYDAFLVTNMVPMYPAFKRVWNYFQRVLVKKYASERNGVNVISGPIFDYDYDGLHDTEDKIKQYV EGSSIPVPTHYYSIITSCLDFTQPADKCDGPLSVSSFILPHRPDNEESCNSSEDESKWVEELMKMHTARVRDIE HLTSLDFFRKTSRSYPEILTLKTYLHTYESEIGGHHHHHHHH
• Purity: ≥90%, as determined by SDS-PAGE
• Usage: FOR RESEARCH ONLY
• Storage: The protein sample can be stored under sterile conditions at 2- 8 centigrade for one month or at -70 centigrade for three months without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Gene Information

• Gene Name: ENPP2 ectonucleotide pyrophosphatase/phosphodiesterase 2 [ Homo sapiens ]
• Official Symbol: ENPP2
• Synonyms: ATX; NPP2; ATX-X; PDNP2; LysoPLD; AUTOTAXIN; PD-IALPHA; ectonucleotide pyrophosphatase/phosphodiesterase family member 2; E-NPP 2; autotaxin-t; extracellular lysophospholipase D; phosphodiesterase I/nucleotide pyrophosphatase 2; plasma lysophospholipase D
• Gene ID: 5168
• mRNA Refseq: NM_001040092
• Protein Refseq: NP_001035181
• MIM: 601060
• UniProt ID: Q13822
• Chromosome Location: 8q24.1
• Pathway: Ether lipid metabolism, organism-specific biosystem; Ether lipid metabolism, conserved biosystem
• Function: alkylglycerophosphoethanolamine phosphodiesterase activity; calcium ion binding; hydrolase activity