Active Recombinant Human ENTPD3 protein, His-tagged

• Cat.No.: ENTPD3-208H
• Product Overview: Active Recombinant Human ENTPD3 protein(O75355)(Gln 44- 485) was expressed in Insect Cells, fused with a polyhistidine tag at the C-terminus and a signal peptide at the N-terminus.

Specification

• Species: Human
• Source: Insect Cells
• Tag: His
• Protein Length: 44-485 aa
• Form: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% gly.
• Bio-activity: Measured by its ability to hydrolyze the 5’-phosphate groups from the substrate adenosine-5’-triphosphate (ATP). The orthophosphate duct is measured by a Malachite Green Phosphate Detection Kit. The specific activity is >70,000 pmol/min/μg.
• Molecular Mass: The recombinant human ENTPD3 (aa44-485) consists of 452 amino acids and predicts a molecular mass of 51 kDa. It migrates as an apximately 50 KDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 97 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• protein Refseq-Weblink: http://www.ncbi.nlm.nih.gov/protein/NP_001239.2
• Unit ID: O75355
• Unit ID-Weblink: http://www.uniprot.org/uniprot/O75355

Gene Information

• Gene Name: ENTPD3 ectonucleoside triphosphate diphosphohydrolase 3 [ Homo sapiens ]
• Official Symbol: ENTPD3
• Synonyms: ENTPD3; ectonucleoside triphosphate diphosphohydrolase 3; CD39L3; HB6; NTPDase 3; CD39-like 3; ecto-ATPase 3; ecto-ATPDase 3; ecto-apyrase 3; CD39 antigen-like 3; ecto-ATP diphosphohydrolase 3; NTPDase-3; FLJ93839
• Gene ID: 956
• mRNA Refseq: NM_001248
• protein Refseq: NP_001239
• MIM: 603161