Active Recombinant Human EPHA7 protein, His-tagged

• Cat.No.: EPHA7-3228H
• Product Overview: Active Recombinant Human EPHA7 protein(NP_004431.1)(Met 1-Ile 556) was expressed in HEK293, fused with a polyhistidine tag at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 1-556 aa
• Form: Lyophilized from sterile PBS, pH 7.4.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized human EphA7 at 2 μg/ml (100 μl/well) can bind mouse EphrinA4 with a linear range of 0.08-10 ng/ml.
• Molecular Mass: The recombinant human EPHA7 consists of 540 amino acids and has a predicted molecular mass of 60.5 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh EPHA7 is approximately 70-80 kDa due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 98 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: EPHA7 EPH receptor A7 [ Homo sapiens ]
• Official Symbol: EPHA7
• Synonyms: EPHA7; EPH receptor A7; EphA7; ephrin type-A receptor 7; Hek11; EK11; EHK-3; EPH-like kinase 11; EPH homology kinase 3; Eph homology kinase-3; receptor protein-tyrosine kinase HEK11; tyrosine-protein kinase receptor EHK-3; EHK3; HEK11
• Gene ID: 2045
• mRNA Refseq: NM_004440
• Protein Refseq: NP_004431
• MIM: 602190
• UniProt ID: Q15375