Active Recombinant Human FCGR3A protein, His-tagged
Cat.No. : | FCGR3A-309H |
Product Overview : | Recombinant Human FCGR3A protein(P08637-1)(Met1-Gln208,F176V), fused with His tag, was expressed in HEK293. |
- Specification
- Gene Information
- Related Products
Source : | HEK293 |
Species : | Human |
Tag : | His |
Form : | Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA. |
Bio-activity : | 1.Immobilized Human FcγRIIIA / CD16a (V176) recombinant protein at 2 μg/mL can bind Human IgG1, the EC50 is 150-450 ng/mL.2.Loaded Human FcγRIIIA / CD16a (V176) recombinant protein on His1K Biosensor, can bind Bevacizumab (IgG1) with an affinity constant of 0.83 μM as determined in a BLI assay (ForteBio Octet Red384) .3.Captured Human FcγRIIIA / CD16a (V176) recombinant protein on Anti-His Chip can bind Bevacizumab (IgG1) with an affinity constant of 0.31 μM as determined in an SPR assay (Biacore T200).4.Captured Bevacizumab (IgG1) on proA Chip can bind Human FcγRIIIA / CD16a (V176) recombinant protein with an affinity constant of 0.78 µM as determined in an SPR assay (Biacore T200). |
Molecular Mass : | The secreted recombinant human CD16a consists of 203 amino acids and predicts a molecular mass of 23.3 KDa. By SDS-PAGE under reducing conditions, the apparent molecular mass of human CD16a is approximately 44.2 KDa due to glycosylation. |
Proteinlength : | Met1-Gln208,F176V |
Endotoxin : | < 1.0 EU per μg of the protein as determined by the LAL method |
Purity : | > 95 % as determined by SDS-PAGE. > 95 % as determined by SEC-HPLC |
Storage : | Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
Gene Name : | FCGR3A Fc fragment of IgG, low affinity IIIa, receptor (CD16a) [ Homo sapiens ] |
Official Symbol : | FCGR3A |
Synonyms : | FCGR3A; Fc fragment of IgG, low affinity IIIa, receptor (CD16a); Fc fragment of IgG, low affinity IIIa, receptor for (CD16) , FCG3, FCGR3; low affinity immunoglobulin gamma Fc region receptor III-A; CD16; CD16a; FcgammaRIIIA; CD16a antigen; fc-gamma RIII; Fc-gamma RIIIa; Fc-gamma RIII-alpha; igG Fc receptor III-2; Fc gamma receptor III-A; Fc-gamma receptor IIIb (CD16); neutrophil-specific antigen NA; Fc-gamma receptor III-2 (CD 16); immunoglobulin G Fc receptor III; Fc fragment of IgG, low affinity III, receptor for (CD16); FCG3; CD16A; FCGR3; IGFR3; FCR-10; FCRIII; FCGRIII; FCRIIIA; |
Gene ID : | 2214 |
mRNA Refseq : | NM_000569 |
Protein Refseq : | NP_000560 |
MIM : | 146740 |
UniProt ID : | P08637 |
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◆ Lysates | ||
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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