Active Recombinant Human FCGR3B protein, His & Avi-tagged, Biotinylated

Cat.No. : FCGR3B-353H
Product Overview : Biotinylated Human FCGR3B is expressed from human HEK293 cells. It contains AA Gly 17 - Ser 200 (Accession # AAI28563). Biotinylation of this product is performed using Avitag technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin. The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
  • Specification
  • Gene Information
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Source : HEK293
Species : Human
Tag : His & Avi
Form : Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Bio-activity : Biotinylated Human CD16b (NA2), His,Avitag captured on Biotin CAP- Series S Sensor Chip can bind Rituximab with an affinity constant of 4.22 μM as determined in a SPR assay (Biacore T200).
Molecular Mass : The protein has a calculated MW of 24.5 kDa. The protein migrates as 37-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Protein Length : Gly 17 - Ser 200
Endotoxin : Less than 1.0 EU per μg by the LAL method.
Purity : >95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Storage : For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 centigrade to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Gene Name : FCGR3B Fc fragment of IgG, low affinity IIIb, receptor (CD16b) [ Homo sapiens ]
Official Symbol : FCGR3B
Synonyms : FCGR3B; Fc fragment of IgG, low affinity IIIb, receptor (CD16b); Fc fragment of IgG, low affinity IIIb, receptor for (CD16) , FCG3, FCGR3; low affinity immunoglobulin gamma Fc region receptor III-B; CD16; CD16b; fc-gamma RIII; fc-gamma RIIIb; fc-gamma RIII-beta; igG Fc receptor III-1; Fc-gamma receptor IIIb (CD 16); Fc fragment of IgG, low affinity IIIb, receptor for (CD16); FCG3; FCGR3; FCR-10; FCRIII; FCRIIIb;
Gene ID : 2215
mRNA Refseq : NM_000570
Protein Refseq : NP_000561
MIM : 610665
UniProt ID : O75015

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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