Active Recombinant Human FCGRT & B2M protein, His-Avi-tagged
Cat.No. : | FCGRT & B2M-1537H |
Product Overview : | Recombinant Human Fcgrt protein(Met1-Ser297)(NP_004098.1) was fused with a C-terminal polyhistidine tagged AVI tag at the C-terminus, constructed the plasmid 1; Recombinant Rat B2M protein (NP_004039.1) (Met1-Met119) constructed the plasmid 2. The two plasmids were co-expressed and the human FCGRT/B2M heterodimer was purified. The purified heterodimer was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed. |
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Source : | HEK293 |
Species : | Human |
Tag : | His-Avi |
Form : | Lyophilized from sterile PBS, pH 7.4 |
Bio-activity : | 1. Measured by its ability to bind human IgG1 (Fc) in a functional ELISA. 2.Loaded Biotinylated Human FcRn recombinant protein (His & Avi Tag) on SA Biosensor, can bind IgG1 Fc (IgG1) with an affinity constant of 0.073 μM as determined in a BLI assay (ForteBio Octet Red384). 3.Loaded Biotinylated Human FcRn recombinant protein (His & Avi Tag) on SA Biosensor, can bind IgG4 Fc with an affinity constant of 0.070 μM as determined in a BLI assay (ForteBio Octet Red384). 4.The purity of Human FCGRT & B2M Heterodimer Protein (His & AVI Tag), Biotinylated is more than 95% and the molecular weight of this protein is around 46-56 kDa verified by SEC-MALS. |
Molecular Mass : | The recombinant heterodimer of human FCGRT/B2M comprises 399 (300+99) amino acids and has a calculated molecular mass of 45.3 (33.6+11.7) KDa. |
Protein Length : | Met1-Ser297&Met1-Met119 |
Endotoxin : | < 1.0 EU per μg of the protein as determined by the LAL method |
Purity : | > 95 % as determined by SDS-PAGE. > 90 % as determined by SEC-HPLC |
Storage : | Samples are stable for up to twelve months from date of receipt at -20°C to -80°C Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
Products Types
◆ Recombinant Protein | ||
FCGRT-1963R | Recombinant Rat FCGRT Protein, His (Fc)-Avi-tagged | +Inquiry |
Fcgrt-2975M | Recombinant Mouse Fcgrt Protein, Myc/DDK-tagged | +Inquiry |
FCGRT-1210H | Recombinant Human FCGRT Protein, GST-tagged | +Inquiry |
FCGRT-1500R | Recombinant Rhesus Macaque FCGRT Protein, His (Fc)-Avi-tagged | +Inquiry |
FCGRT-3998H | Recombinant Human FCGRT Protein, GST-tagged | +Inquiry |
◆ Lysates | ||
FCGRT-6278HCL | Recombinant Human FCGRT 293 Cell Lysate | +Inquiry |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Ask a questionCustomer Reviews (3)
Write a reviewWith a long shelf life, a single bottle of the reagent can be used multiple times, which is especially valuable for budget-conscious laboratories.
It maintains its activity and effectiveness under various environmental conditions.
The reagent has a wide range of applications, catering to diverse needs in different laboratories, be it in biomedical research or the field of bioengineering.
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