Active Recombinant Human FN1, His-tagged

• Cat.No.: FN1-519H
• Product Overview: Recombinant Human FN1 (Accession # CAE45932) Met1-Pro1908, fused with a C-terminal 6-His tag, was produced in Spodoptera frugiperda, Sf 9 (baculovirus)-derived.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: His
• Protein Length: 1-1908 a.a.
• Predicted N Terminal: No results obtained: Gln32 predicted, sequencing might be blocked
• Form: Lyophilized from a 0.2 μm filtered solution in PBS and Tween® 20.
• Bio-activity: Measured by its ability to support cell attachment and spreading when used as a substratum for cell culture.In this application, the recommended concentration for this effect is typically 1 - 5 μg/cm2.Fibronectin can also be added to the media to support cell spreading at a concentration of 0.5 - 50 μg/mL.
• Molecular Mass: Recombinant Human FN1, His-tagged has a calculated MW of 206.7 kDa. In SDS-PAGE migrates as 190-210 kDa, reducing conditions.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: FN1 fibronectin 1 [ Homo sapiens ]
• Official Symbol: FN1
• Synonyms: FN1; fibronectin 1; fibronectin; CIG; cold insoluble globulin; FINC; GFND2; LETS; migration stimulating factor; MSF; cold-insoluble globulin; migration-stimulating factor; FN; FNZ; ED-B; GFND; DKFZp686H0342; DKFZp686I1370; DKFZp686F10164; DKFZp686O13149
• Gene ID: 2335
• mRNA Refseq: NM_002026
• Protein Refseq: NP_002017
• MIM: 135600
• UniProt ID: P02751
• Chromosome Location: 2q34