Active Recombinant Human GFRA3, Fc Chimera

• Cat.No.: GFRA3-237H
• Product Overview: Recombinant Human GFRA3 (Accession # AAC24355) was produced in Spodoptera frugiperda, Sf 21 (baculovirus)-derived.

Specification

• Species: Human
• Source: Sf21 Cells
• Tag: Fc
• Predicted N Terminal: Gly31 & Asp32
• Form: Lyophilized from a 0.2 µm filtered solution in PBS.
• Bio-activity: Binding of R&D Systems" human GFR alpha -3/Fc Chimera to immobilized Artemin has been reported. Baloh, R. et al. (1998) Neuron 21:1291.80 ng/mL of the rhMSP significantly induces phosphorylation of MSP R/Ron measured by DuoSet IC human phospho-MSP R/Ron kit. Measured by its binding ability in a functional ELISA.Immobilized Recombinant Human Artemin at 1 µg/mL can bind Recombinant Human GFR alpha -3/GDNF R alpha -3 Fc Chimera with an apparent Kd <1 nM.
• Molecular Mass: Recombinant Human GFRA3, Fc Chimera has a calculated MW of 67 kDa (monomer). In SDS-PAGE migrates as 67 kDa, reducing conditions.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: GFRA3 GDNF family receptor alpha 3 [ Homo sapiens ]
• Official Symbol: GFRA3
• Synonyms: GFRA3; GDNF family receptor alpha 3; GDNF family receptor alpha-3; GFRa 3; GFR-alpha-3; GDNFR-alpha-3; GPI-linked receptor; GDNF receptor alpha-3; glial cell line-derived neurotrophic factor receptor alpha-3
• Gene ID: 2676
• mRNA Refseq: NM_001496
• Protein Refseq: NP_001487
• MIM: 605710
• UniProt ID: O60609
• Chromosome Location: 5q31.1-q31.3