Active Recombinant Human GPRC5D protein, His&Flag-tagged, Biotinylated

• Cat.No.: GPRC5D-506H
• Product Overview: Biotinylated Recombinant Human GPRC5D protein(Q9NZD1-2)(Tyr 2 - Phe 192 & Glu 197 - Leu 262), fused with C-terminal His tag and N-terminal Flag tag, was expressed from HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Flag&His
• Protein Length: Tyr 2 - Phe 192 & Glu 197 - Leu 262
• Form: Supplied as 0.2 μm filtered solution in 50 mM HEPES, 150 mM NaCl, DDM, CHS, pH7.5 with glycerol as protectant.
• Bio-activity: Immobilized Human GPRC5D, Flag,His Tag at 10 μg/mL (100 μL/well) on an Nickel Coated plate can bind Anti-GPRC5D antibody with a linear range of 2-78 ng/mL.
• Molecular Mass: The protein has a calculated MW of 51.8 kDa. The protein migrates as 35-38 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >85% as determined by SDS-PAGE.
• Storage: Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20°C to -70°C for 12 months in lyophilized state;
  -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Conjugation: Biotin

Gene Information

• Gene Name: GPRC5D G protein-coupled receptor, family C, group 5, member D [ Homo sapiens ]
• Official Symbol: GPRC5D
• Synonyms: GPRC5D; G protein-coupled receptor, family C, group 5, member D; G-protein coupled receptor family C group 5 member D; orphan G-protein coupled receptor; MGC129713; MGC129714
• Gene ID: 55507
• mRNA Refseq: NM_018654
• Protein Refseq: NP_061124
• MIM: 607437
• UniProt ID: Q9NZD1