Active Recombinant Human Growth Arrest-Specific 1, His-tagged

• Cat.No.: GAS1-609H
• Product Overview: RecombinantHuman GAS1 protein was expressed in Murinemyeloma cell line. Leu40-Ser318, with a C-terminal 6-Histag.

Specification

• Species: Human
• Source: Mammalian Cells
• Tag: His
• Protein Length: 40-318 a.a.
• Description: GAS1 is one of six structurally unrelated proteins thatwere identified by their increased expression in growth-arrested cells relativeto actively proliferating cells. Following mitogenic stimulation, GAS1 expression is transcriptionally suppressed by c-Myc ascells transit from G 0 to G1 phases of the cell cycle. Overexpression of GAS1 prevents S phase entry and DNA synthesis. GAS1-mediated blockade of the cell cycle is p53-dependent butdoes not require the transactivating domain of p53. The human GAS1 cDNA encodes a 345 amino acid (aa) precursor thatincludes a 39 aa signal sequence, a 279 aa mature protein, and a 27 aa C-terminalpropeptide. GAS1 contains Ala-rich and Asp-rich regions as well as anRGD sequence.
• Form: Supplied as a 0.2 umfiltered solution in Tris and NaCl.
• N-terminalSequence: Leu40
• MolecularWeight: 30.1 kDa
• Activity: Measuredby its binding ability in a functional ELISA. Immobilized rhGas1 at 1 μg/mL(10μL/well) can bind rmArtemin at a linear range of 0.1-10 ng/mL.
• Endotoxin Level: <1.0EU per 1μg of the protein by the LAL method.
• SDS-PAGE: 40 kDa,reducing conditions.
• Purity: >95%,by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution: Reconstituteat 100μg/mL in sterile PBS.
• Storage: Use amanual defrost freezer and avoid repeated freezethaw cycles. 6 monthsfrom date of receipt, -20 to -70°C as supplied. 3 months,-20to-70°C under sterile conditions after opening.

Gene Information

• Gene Name: GAS1 growth arrest-specific 1 [ Homosapiens ]
• Official Symbol: GAS1
• Synonyms: GAS1; growth arrest-specific 1;growth arrest-specific protein 1; Growth arrest specific gene 1;Growth-arrest-specific protein 1 precursor; GAS-1; OTTHUMP00000021599; Growtharrest-specific gene-1
• Gene ID: 2619
• mRNA Refseq: NM_002048
• Protein Refseq: NP_002039
• MIM: 139185
• UniProt ID: P54826
• Chromosome Location: 9q21.3-q22
• Pathway: FOXM1 transcriptionfactor network; Hedgehog Signaling Pathway; Signaling events mediated by theHedgehog family
• Function: protein binding