Active Recombinant Human HHLA2 protein, His-Avi-tagged, Biotinylated

• Cat.No.: HHLA2-2897H
• Product Overview: Biotinylated Recombinant Human HHLA2 protein(Ile 23-Asn 344), fused with His and Avi tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: His&Avi
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Biotinylated Human B7-H7, His,Avitag at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate can bind Human CD28H, Fc Tag with a linear range of 0.1-3 ng/mL.Immobilized Human CD28H, Fc Tag at 5 μg/mL (100 μL/well) can bind Biotinylated Human B7-H7, His,Avitag with a linear range of 0.039-1.25 μg/mL.
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).The protein has a calculated MW of 40.7 kDa. The protein migrates as 50-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Protein length: Ile 23-Asn 344
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE. >90% as determined by SEC-MALS.
• Storage: For long term storage, the product should be stored at lyophilized state at-20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after storage at:-20°C to-70°C for 12 months in lyophilized state;-70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: HHLA2 HERV-H LTR-associating 2 [ Homo sapiens ]
• Official Symbol: HHLA2
• Synonyms: HHLA2; HERV-H LTR-associating 2; HERV-H LTR-associating protein 2; human endogenous retrovirus-H long terminal repeat-associating protein 2
• Gene ID: 11148
• mRNA Refseq: NM_007072
• Protein Refseq: NP_009003
• MIM: 604371
• UniProt ID: Q9UM44