Active Recombinant Human HSD17B13 Protein, C-DDK-tagged

• Cat.No.: HSD17B13-07H
• Product Overview: Purified Biotinylated recombinant human hydroxysteroid (17-beta) dehydrogenase 13 (HSD17B13) with C-terminal DDK tag and site-specific Biotinylation on the C-terminal AVIplus tag was expressed in HEK293T.

Specification

• Description: Predicted to enable oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor and steroid dehydrogenase activity. Acts upstream of or within positive regulation of lipid biosynthetic process. Located in lipid droplet.
• Source: HEK293T
• Species: Human
• Tag: C-DDK
• Bio-activity: The biotin to protein ratio is about 0.2 as determined by Streptavidin Pull-Down Assay.
• Molecular Mass: 36.8 kDa
• AA Sequence: MNIILEILLLLITIIYSYLESLVKF FIPQRRKSVAGEIVLITGAGHGIGR QTTYEFAKRQSILVLWDINKRGVEE TAAECRKLGVTAHAYVVDCSNREEI YRSLNQVKKEVGDVTIVVNNAGTVY PADLLSTKDEEITKTFEVNILGHFW ITKALLPSMMERNHGHIVTVASVCG HEGIPYLIPYCSSKFAAVGFHRGLT SELQALGKTGIKTSCLCPVFVNTGF TKNPSTRLWPVLETDEVVRSLIDGI LTNKKMIFVPSYINIFLRLQKFLPE RASAILNRMQNIQFEAVVGHKIKMK TRTRPLEQKLISEEDLAANDILDYK DDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Applications: Cell culture: For culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 μg/mL as determined by Bradford protein assay method.
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

Gene Information

• Gene Name: HSD17B13 hydroxysteroid 17-beta dehydrogenase 13 [ Homo sapiens (human) ]
• Official Symbol: HSD17B13
• Synonyms: HSD17B13; hydroxysteroid 17-beta dehydrogenase 13; FLDP; SCDR9; NIIL497; SDR16C3; HMFN0376; 17-beta-hydroxysteroid dehydrogenase 13; 17-beta hydroxysteroid dehydrogenase; 17-beta-HSD 13; short chain dehydrogenase/reductase family 16C member 3; short-chain dehydrogenase/reductase 9
• Gene ID: 345275
• mRNA Refseq: NM_178135
• Protein Refseq: NP_835236
• MIM: 612127
• UniProt ID: Q7Z5P4