Active Recombinant Human HTRA2 protein, His-tagged

• Cat.No.: HTRA2-968H
• Product Overview: Active Recombinant Human HTRA2 protein(O43464-1) (Ala 134-Glu 458) was expressed in E. coli, with a polyhistide tag at the C-terminus.

Specification

• Species: Human
• Source: E. coli
• Tag: His
• Protein Length: 134-458 aa
• Form: Lyophilized from sterile 50mM Tris, 0.3M NaCl, 1mM DTT, 20% Glycerol, pH 7.8.
• Bio-activity: Protease activity demonstrated by HtrA2 cleavage of bovine β-casein. Incubation of β-casein at 0.2 mg/mL with Recombinant Human HTRA-2 at 0.02 mg/mL (ratio of 10:1) for 60 minutes at 45°C in 50 mM Tris, pH 8.0, which results in >95% cleavage of β-casein, as revealed by SDS-PAGE.
• Molecular Mass: The recombinant human HTRA2 consisting of 336 amino acids and has a calculated molecular mass of 36.5 kDa as estimated in SDS-PAGE under reducing conditions.
• Purity: > 87 % by SDS-PAGE and HPLC analyses.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 °C as supplied. - 1 month, 2 to 8 °C under sterile conditions after reconstitution. - 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C.

Gene Information

• Gene Name: HTRA2 HtrA serine peptidase 2 [ Homo sapiens ]
• Official Symbol: HTRA2
• Synonyms: HTRA2; HtrA serine peptidase 2; protease, serine, 25 , PRSS25; serine protease HTRA2, mitochondrial; OMI; PARK13; serine protease 25; protease, serine, 25; serine proteinase OMI; HtrA-like serine protease; Omi stress-regulated endoprotease; high temperature requirement protein A2; PRSS25
• Gene ID: 27429
• mRNA Refseq: NM_013247
• Protein Refseq: NP_037379
• MIM: 606441
• UniProt ID: O43464