Active Recombinant Human ICAM2 protein, His-tagged

• Cat.No.: ICAM2-2170H
• Product Overview: Recombinant Human ICAM2 protein(NP_000864.2)(Met1-Gln223), fused with His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Met1-Gln223
• Form: Lyophilized from sterile PBS, pH 7.5Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells. When cells are added to ICAM2-coated plates (12.5 μg/ml, 100 μl/well), approximately 35 %-45% will adhere specifically.
• Molecular Mass: The recombinant human ICAM2 consists of 213 amino acids after removal of the signal peptide and has a predicted molecular mass of 24 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhICAM2 is approximately 50-55 kDa due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 97 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: ICAM2 intercellular adhesion molecule 2 [ Homo sapiens ]
• Official Symbol: ICAM2
• Synonyms: ICAM2; intercellular adhesion molecule 2; CD102; ICAM-2
• Gene ID: 3384
• mRNA Refseq: NM_000873
• Protein Refseq: NP_000864
• MIM: 146630
• UniProt ID: P13598