Species : |
Human |
Source : |
HEK293 |
Tag : |
Fc&His |
Protein Length : |
Met1-Gln223 |
Form : |
Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. |
Bio-activity : |
Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells. When cells are added to ICAM2-coated plates (12.5 μg/mL, 100 μL/well), approximately 50 % - 60 % will adhere specifically. |
Molecular Mass : |
The recombinant human ICAM2/Fc is a disulfide-linked homodimer after removal of the signal peptide. The reduced monomer consists of 449 amino acids and has a predicted molecular mass of 50.3 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhICAM2/Fc monomer is approximately 85-95 kDa due to glycosylation. |
Endotoxin : |
< 1.0 EU per μg of the protein as determined by the LAL method. |
Purity : |
> 97 % as determined by SDS-PAGE |
Storage : |
Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |