| Species : |
Human |
| Source : |
E.coli |
| Tag : |
His |
| Protein Length : |
2-403 (full length) |
| Description : |
This gene encodes indoleamine 2,3-dioxygenase (IDO) - a heme enzyme that catalyzes the first and rate-limiting step in tryptophan catabolism to N-formyl-kynurenine. This enzyme acts on multiple tryptophan substrates including D-tryptophan, L-tryptophan, 5-hydroxy-tryptophan, tryptamine, and serotonin. This enzyme is thought to play a role in a variety of pathophysiological processes such as antimicrobial and antitumor defense, neuropathology, immunoregulation, and antioxidant activity. Through its expression in dendritic cells, monocytes, and macrophages this enzyme modulates T-cell behavior by its peri-cellular catabolization of the essential amino acid tryptophan. |
| Form : |
40 mM Tris-HCl, pH 8.0, with 110 mM sodium chloride, 2.2 mM potassium chloride, and 20% glycerol. |
| Bio-activity : |
IDO1 activity is measured by incubating with L-Trp substrate. After a three hour roomtemperature incubation, activity is determined by measuring the absorption of reaction product at λ = 320-325 nm. |
| Molecular Mass : |
46 kDa |
| Purity : |
≥90% estimated by SDS-PAGE |
| Applications : |
Enzyme Activity |
| Stability : |
≥ 6 months |
| Storage : |
-80 centigrade |
| Shipping : |
Dry ice in continental US; may vary elsewhere |
