Active Recombinant Human IFNA1 Protein (Cys24-Glu189), N-His tagged, Animal-free, Carrier-free

• Cat.No.: IFNA1-154H
• Product Overview: Recombinant Human IFNA1 Protein (Cys24-Glu189) with N-His tag was expressed in E. coli and Animal-free as well as Carrier-free.

Specification

• Description: Interferon-alpha 1a (IFN-alpha 1a) is a leukocyte interferon, which is a variant of Interferon-alpha. IFN-alpha 1a is a 19.5kDa protein containing 167 amino acid residues. It could bind the interferon receptors that activates the signal transduction of immune responses through the Jak/STAT pathway in leukocytes and lymphoblastoid cells.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Lyophilized
• Bio-activity: Measure by its ability to inhibit IL-8 secretion in human PBMCs in the presence of LPS. The ED50 for this effect is < 1.12 μg/mL.
• Molecular Mass: The protein has a calculated MW of 20.19 kDa.
  The protein migrates as 19 kDa under reducing condition (SDS-PAGE analysis).
• Protein length: Cys24-Glu189
• Endotoxin: < 0.1 EU/μg of the protein by the LAL method.
• Purity: >95% as determined by SDS-PAGE analysis.
• Storage: This product is stable after storage at:
  -20 centigrade for 12 months in lyophilized state from date of receipt.
  -20 or -80 centigrade for 1 month under sterile conditions after reconstitution.
  Avoid repeated freeze/thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 8.0.
• Reconstitution: It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
• Shipping: The product is shipped with polar packs. Upon receipt, store it immediately at -20 centigrade or lower for long term storage.

Gene Information

• Gene Name: IFNA1 interferon, alpha 1 [ Homo sapiens (human) ]
• Official Symbol: IFNA1
• Synonyms: IFNA1; interferon, alpha 1; interferon alpha-1/13; IFL; IFN; IFN ALPHA; IFN alpha 1b; IFN alphaD; IFNA13; IFNA@; interferon alpha 1b; leIF D; IFN-alpha 1b; IFN-alpha-1/13; interferon-alpha1; interferon alpha-D; IFN-ALPHA; IFN-alphaD; MGC138207; MGC138505; MGC138507
• Gene ID: 3439
• mRNA Refseq: NM_024013
• Protein Refseq: NP_076918
• MIM: 147660
• UniProt ID: P01562