Active Recombinant Human IGHE protein, His-tagged

• Cat.No.: IGHE-696H
• Product Overview: Recombinant Human IGHE protein(Ser 106 - Gly 427), fused with His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 106-427 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
• Bio-activity: Cynomolgus Fc epsilon RI alpha, His Tag immobilized on CM5 Chip can bind Human IgE Fc, His Tag with an affinity constant of 0.226 nM as determined in a SPR assay (Biacore 8K).Human Fc epsilon RI alpha, Fc Tag immobilized on CM5 Chip can bind Human IgE Fc, His Tag with an affinity constant of 0.585 nM as determined in a SPR assay (Biacore 8K).
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminusThe protein has a calculated MW of 37.6 kDa. The protein migrates as 45-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Ser106-Gly427
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: IGHE Immunoglobulin heavy constant epsilon [ Homo sapiens ]
• Official Symbol: IGHE
• Synonyms: IGHE; Immunoglobulin heavy constant epsilon
• Gene ID: 28223