| Species : |
Human |
| Source : |
HEK293 |
| Tag : |
His |
| Protein Length : |
106-428 aa |
| Form : |
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Bio-activity : |
Immobilized Human Fc epsilon RI alpha, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Human IgE, His Tag with the EC50 of 2.5ng/ml determined by ELISA. |
| Molecular Mass : |
The protein has a predicted MW of 37.49 kDa. Due to glycosylation, the protein migrates to 45-60 kDa based on Bis-Tris PAGE result. |
| Endotoxin : |
Less than 0.01 EU per μg by the LAL method. |
| Purity : |
> 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC |
| Storage : |
Store at -20 to -80°C for 24 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
| Reconstitution : |
Dissolve the lyophilized protein in distilled water. |
