Active Recombinant Human IL17RA&IL17RC protein, Fc-tagged

• Cat.No.: IL17RA&IL17RC-1628H
• Product Overview: Recombinant Human IL17RA&IL17RC protein(Leu33-Trp320(IL-17RA)&Leu21-His465(IL-17RC)), fused with Fc tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Protein Length: 33-320;21-465 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
• Bio-activity: Immobilized Human IL-17A&IL-17F Heterodimer Protein, His Tag&Strep II Tag at 2 μg/mL (100 μL/well) can bind Human IL17RA&IL17RC Protein, Fc Tag&Fc Tag with a linear range of 1-20 ng/mL.Immobilized Human IL17A, Tag Free at 2 μg/mL (100 μL/well) can bind Human IL17RA&IL17RC Protein, Fc Tag&Fc Tag with a linear range of 0.6-10 ng/mL.Immobilized Human IL-17F (H161R), His Tag at 2 μg/mL (100 μL/well) can bind Human IL17RA&IL17RC Protein, Fc Tag&Fc Tag with a linear range of 1-39 ng/mL.
• Molecular Mass: Human IL17RA&IL17RC Protein, Fc Tag&Fc Tag is produced by co-expression of IL-17RA and IL-17RC, has a calculated MW of 60.0 kDa (IL-17RA) & 75.7 kDa (IL-17RC). Subunit IL-17RA is fused with a human IgG1 Fc tag at the C-terminus and subunit IL-17RC is fused with a human IgG1 Fc tag at the C-terminus. The reducing (R) heterodimer protein migrates as 80-90 kDa and 100-110 kDa due to glycosylation.
• Proteinlength: Leu33-Trp320(IL-17RA)&Leu21-His465(IL-17RC)
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE. >90% as determined by SEC-MALS.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: IL17RA interleukin 17 receptor A [ Homo sapiens ]
• Official Symbol: IL17RA
• Synonyms: IL17RA; interleukin 17 receptor A; IL17R, interleukin 17 receptor; interleukin-17 receptor A; CD217; CDw217; hIL 17R; IL 17RA; IL-17 receptor A; IL17R; CANDF5; IL-17RA; hIL-17R; FLJ32274; FLJ36453; MGC10262
• Gene ID: 23765
• mRNA Refseq: NM_014339
• Protein Refseq: NP_055154
• MIM: 605461
• UniProt ID: Q96F46