Active Recombinant Human IMPDH2 Protein, His tagged

• Cat.No.: IMPDH2-01H
• Product Overview: Recombinant Human IMPDH2 Protein with His tag was expressed in Baculovirus-Insect Cells.

Specification

• Species: Human
• Source: Insect Cells
• Tag: His
• Description: This gene encodes the rate-limiting enzyme in the de novo guanine nucleotide biosynthesis. It is thus involved in maintaining cellular guanine deoxy- and ribonucleotide pools needed for DNA and RNA synthesis. The encoded protein catalyzes the NAD-dependent oxidation of inosine-5'-monophosphate into xanthine-5'-monophosphate, which is then converted into guanosine-5'-monophosphate. This gene is up-regulated in some neoplasms, suggesting it may play a role in malignant transformation.
• Bio-activity: Measured by its ability to convert the substrate inosine ­5' ­phosphate (IMP) to xanthosine ­5' ­phosphate (XMP). The specific activity is 136 pmol/min/μg as measured under the described conditions.
• Molecular Mass: The protein has a calculated MW of 57 kDa.
• AA Sequence: MADYLISGGTSYVPDDGLTAQQLFNCGDGLTYNDFLILPGYIDFTADQVDLTSALTKKITLKTPLVSSPMDTVTEAGMAIAMALTGGIGFIHHNCTPEFQANEVRKVKKYEQGFITDPVVLSPKDRVRDVFEAKARHGFCGIPITDTGRMGSRLVGIISSRDIDFLKEEEHDCFLEEIMTKREDLVVAPAGITLKEANEILQRSKKGKLPIVNEDDELVAIIARTDLKKNRDYPLASKDAKKQLLCGAAIGTHEDDKYRLDLLAQAGVDVVVLDSSQGNSIFQINMIKYIKDKYPNLQVIGGNVVTAAQAKNLIDAGVDALRVGMGSGSICITQEVLACGRPQATAVYKVSEYARRFGVPVIADGGIQNVGHIAKALALGASTVMMGSLLAATTEAPGEYFFSDGIRLKKYRGMGSLDAMDKHLSSQNRYFSEADKIKVAQGVSGAVQDKGSIHKFVPYLIAGIQHSCQDIGAKSLTQVRAMMYSGELKFEKRTSSAQVEGGVHSLHSYEKRLFHHHHHHHHHH
• Endotoxin: < 0.1 EU/μg by LAL
• Purity: > 90% by SDS-PAGE
• Storage: Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Concentration: 0.33 mg/mL by Bradford
• Storage Buffer: Sterile 20 mM Tris, 0.5M NaCl, 10% glycerol, 2mM DTT, pH 8.0

Gene Information

• Gene Name: IMPDH2 IMP (inosine 5-monophosphate) dehydrogenase 2 [ Homo sapiens ]
• Official Symbol: IMPDH2
• Synonyms: IMPDH2; IMP (inosine 5-monophosphate) dehydrogenase 2; inosine-5-monophosphate dehydrogenase 2; IMPD 2; IMPDH 2; IMP oxireductase 2; inosine 5 phosphate dehydrogenase 2; IMP (inosine monophosphate) dehydrogenase 2; inosine monophosphate dehydrogenase type II; IMPD2; IMPDH-II
• Gene ID: 3615
• mRNA Refseq: NM_000884
• Protein Refseq: NP_000875
• MIM: 146691
• UniProt ID: P12268