Species : |
Human |
Source : |
Mammalian Cells |
Tag : |
Fc |
Protein Length : |
28-570 a.a. |
Description : |
ICAM5 isa cell surface glycoprotein belonging to the immunoglobulin superfamily.Human ICAM5 consists of an 832 amino acid (aa) extracellular domaincontaining 9 immunoglobulin (Ig) domains and 15 N-glycosylation sites, a 28aa transmembrane domain, and a 64 aa cytoplasmic domain. ICAM5 shares 38-55%aa identity with other ICAMs, being most closely related to ICAM-1 (50% identity)and ICAM-3 (55% identity). Human and mouse ICAM5 share 85% aa identity. |
Form : |
Lyophilizedfrom a 0.2 um filtered solution in PBS. |
N-terminalSequence : |
Ala28 |
MolecularWeight : |
85.1 kDa (monomer) |
Activity : |
Measuredby the ability of the immobilized protein to support the adhesion ofPMA-stimulated HSB2 human peripheral blood acute lymphoblastic leukemiacells. When 5x104 cells/well are added to rhICAM5/Fc Chimeracoated plates (25 μg/mL, 100 μL/well), approximately 25-40% will adhere after1 hour incubation at 37°C. |
Endotoxin Level : |
<1.0EU per 1μg of the protein by the LAL method. |
SDS-PAGE : |
125-130kDa, reducing conditions. |
Purity : |
>90%,by SDS-PAGE under reducing conditions and visualized by silver stain. |
Reconstitution : |
400 μg/mLin sterile PBS |
Storage : |
Use a manualdefrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied.1 month, 2 to 8°C under sterile conditions after reconstitution. 3months, -20 to -70°C under sterile conditions after reconstitution. |