Active Recombinant Human IRE1 protein(Pro465-Leu977)

• Cat.No.: IRE1-3230H
• Product Overview: Recombinant Human ERN1 (O75460-1) (Pro 465-Leu 977) was expressed and purified in Insect Cells with two additional amino acids (Gly & Pro ) at the N-terminus.

Specification

• Source: Insect Cells
• Species: Human
• Tag: N/A
• Protein length: Pro465-Leu977
• Form: Supplied as sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 7.4.
• Bio-activity: Measured by its nuclease activity to cleave Xbp1 single stem-loop mini-substrate.
• Molecular Mass: The secreted recombinant human ERN1 consists of 515 amino acids and predicts a molecular mass of 58.3 KDa. The apparent molecular mass of the protein is approximately 65 KDa in SDS-PAGE under reducing conditions due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 80 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Concentration: Please refer to the datasheet for detailed information.