Active Recombinant Human ITGA5&ITGB1 protein, His-tagged

• Cat.No.: ITGA5&ITGB1-1563H
• Product Overview: Recombinant Human ITGA5&ITGB1 protein(Phe42-Tyr995(ITGA5)&Gln21-Asp728(ITGB1)), fused with His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: His
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5.
• Bio-activity: Immobilized Human Fibronectin at 2 μg/mL (100 μL/well) can bind Human ITGA5&ITGB1 Heterodimer Protein, His Tag&Tag Free with a linear range of 0.2-2 ng/mL.
• Molecular Mass: Human ITGA5&ITGB1 Heterodimer Protein, His Tag&Tag Free, produced by co-expression of ITGA5 and ITGB1, has a calculated MW of 110.7 kDa (ITGA5) and 83.7 kDa (ITGB1). Subunit ITGA5 is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB1 contains no tag but a basic tail at the C-terminus. The non-reducing (NR) protein migrates as 130-150 kDa (ITGA5) and 100-120 kDa (ITGB1) respectively due to glycosylation.
• Proteinlength: Phe42-Tyr995(ITGA5)&Gln21-Asp728(ITGB1)
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: ITGA5 integrin, alpha 5 (fibronectin receptor, alpha polypeptide) [ Homo sapiens ]
• Official Symbol: ITGA5
• Synonyms: ITGA5; integrin, alpha 5 (fibronectin receptor, alpha polypeptide); FNRA; integrin alpha-5; CD49e; VLA-5; integrin alpha-F; CD49 antigen-like family member E; fibronectin receptor subunit alpha; fibronectin receptor, alpha subunit; very late activation protein 5, alpha subunit; VLA5A
• Gene ID: 3678
• mRNA Refseq: NM_002205
• Protein Refseq: NP_002196
• MIM: 135620
• UniProt ID: P08648

Reviews

10/15/2022

Outstanding accuracy, accelerates research progress.

08/02/2021

Efficient service, essential for research outcomes.

03/15/2021

Trusted for research integrity, an invaluable research asset.

Q&As

How does ITGA5 interact with other components of the extracellular matrix?

ITGA5 works with extracellular matrix components, regulating cell-matrix interactions.

What is the impact of altered ITGA5 activity on wound healing?

Altered ITGA5 activity can impact wound healing and tissue repair processes.

How do genetic variations in ITGA5 affect cell function?

Genetic mutations in ITGA5 can lead to impaired cell adhesion and migration, affecting tissue integrity.

What is the primary role of ITGA5 in cell adhesion?

ITGA5, an integrin, is crucial for cell adhesion, facilitating cells' attachment to the extracellular matrix.

What role does ITGA5 play in tissue remodeling?

ITGA5 is involved in tissue remodeling, aiding in the reorganization and repair of tissues.

What potential therapeutic applications arise from targeting ITGA5 in regenerative medicine?

Targeting ITGA5 could offer new strategies for enhancing tissue repair and regeneration in various medical conditions.

How does ITGA5 contribute to cell migration?

It plays a key role in cell migration, essential for processes like wound healing and tissue formation.