Active Recombinant Human ITGAV&ITGB5 protein, His-Avi-tagged, Biotinylated
Cat.No. : | ITGAV & ITGB1-7236H |
Product Overview : | Biotinylated Recombinant Human ITGAV&ITGB5 protein(Phe 31 - Val 992 (ITGAV) & Gly 24 - Asn 719 (ITGB5)), fused with His and Avi tag, was expressed in HEK293. |
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Source : | HEK293 |
Species : | Human |
Tag : | His-Avi |
Form : | Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization. |
Bio-activity : | Immobilized Human Vitronectin at 5 μg/mL (100 μL/well) can bind Biotinylated Human ITGAV&ITGB5 Heterodimer Protein, His,Avitag&Tag Free with a linear range of 0.01-0.156 μg/mL.Immobilized Human Fibronectin at 5 μg/mL (100 μL/well) can bind Biotinylated Human ITGAV&ITGB5 Heterodimer Protein, His,Avitag&Tag Free with a linear range of 0.156-5 μg/mL. |
Molecular Mass : | Biotinylated Human ITGAV&ITGB5 Heterodimer Protein, His,Avitag&Tag Free, produced by co-expression of ITGAV and ITGB5, has a calculated MW of 114.7 kDa (ITGAV) and 81.9 kDa (ITGB5). Subunit ITGAV is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and an Avi tag (Avitag™) and subunit ITGB5 contains no tag but a basic tail at the C-terminus. The non-reducing (NR) protein migrates as 140-150 kDa (ITGAV) and 90-98 kDa (ITGB5) respectively due to glycosylation. |
Protein Length : | Phe 31 - Val 992 (ITGAV) & Gly 24 - Asn 719 (ITGB5) |
Endotoxin : | Less than 1.0 EU per μg by the LAL method. |
Purity : | >95% as determined by SDS-PAGE. |
Storage : | For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
Gene Name : | ITGAV integrin, alpha V [ Homo sapiens ] |
Official Symbol : | ITGAV |
Synonyms : | ITGAV; integrin, alpha V; antigen identified by monoclonal L230 , integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51) , MSK8, vitronectin receptor , VNRA, VTNR; integrin alpha-V; CD51; integrin alphaVbeta3; vitronectin receptor subunit alpha; antigen identified by monoclonal L230; integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51); MSK8; VNRA; VTNR; DKFZp686A08142; |
Gene ID : | 3685 |
mRNA Refseq : | NM_001144999 |
Protein Refseq : | NP_001138471 |
MIM : | 193210 |
UniProt ID : | P06756 |
Products Types
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ITGAV-2136R | Recombinant Rhesus Macaque ITGAV Protein, His (Fc)-Avi-tagged | +Inquiry |
Itgav&Itgb6-23R | Recombinant Rat Itgav&Itgb6 Protein | +Inquiry |
◆ Lysates | ||
ITGAV-877HCL | Recombinant Human ITGAV cell lysate | +Inquiry |
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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