Active Recombinant Human KAT5, GST-tagged

• Cat.No.: KAT5-1350H
• Product Overview: Full-length recombinant human KAT5 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: GST
• Protein Length: Full length
• Description: KAT5 or K (lysine) acetyltransferase 5 is a signaling protein that belongs to the MYST family of histone acetyl transferases (HATs). KAT5 was originally isolated as an HIV-1 TAT-interactive protein that plays an important role in regulating chromatin remodeling, transcription and other nuclear processes by acetylating histone and nonhistone proteins. KAT5 protein is a histone acetylase that is involved in DNA repair and apoptosis and plays an important role in signal transduction. KAT5 acts as a cofactor of TAT that is involved in the regulation of HIV gene expression. KAT5 is also a co-regulator of transcription factors that either promote or suppress tumorigenesis.
• Form: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 50mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Bio-activity: 30 nmol/min/mg
• Molecular Mass: ~88 kDa
• Purity: >90%
• Applications: Acetyltransferase Assay
• Storage: Store at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. Avoid freeze/thaw cycles.
• Concentration: 0.1 μg/μl
• Publications:
  Targeted systematic evolution of an RNA platform neutralizing DNMT1 function and controlling DNA methylation (2023)

Gene Information

• Gene Name: KAT5 K(lysine) acetyltransferase 5 [ Homo sapiens ]
• Official Symbol: KAT5
• Synonyms: KAT5; K(lysine) acetyltransferase 5; HIV 1 Tat interactive protein, 60kDa , HTATIP; histone acetyltransferase KAT5; cPLA2; ESA1; HTATIP1; K acetyltransferase 5; PLIP; Tat interacting protein; 60kDa; TIP60; K-acetyltransferase 5; cPLA2 interacting protein; lysine acetyltransferase 5; cPLA(2)-interacting protein; 60 kDa Tat-interactive protein; Tat interacting protein, 60kDa; histone acetyltransferase HTATIP; HIV-1 Tat interactive protein, 60kDa; TIP; HTATIP
• Gene ID: 10524
• mRNA Refseq: NM_001206833
• Protein Refseq: NP_001193762
• MIM: 601409
• UniProt ID: Q92993
• Chromosome Location: 11q13
• Pathway: ATF-2 transcription factor network, organism-specific biosystem; Androgen Receptor Signaling Pathway, organism-specific biosystem; C-MYC pathway, organism-specific biosystem; HTLV-I infection, organism-specific biosystem; HTLV-I infection, conserved biosystem; IL-9 Signaling Pathway, organism-specific biosystem; Regulation of Androgen receptor activity, organism-specific biosystem
• Function: androgen receptor binding; contributes_to histone acetyltransferase activity; metal ion binding; protein binding; repressing transcription factor binding; transcription coactivator activity; transcription coactivator activity; transferase activity, transferring acyl groups other than amino-acyl groups

Citation

Targeted systematic evolution of an RNA platform neutralizing DNMT1 function and controlling DNA methylation

Journal: bioRxiv    Data: 2020/7/29

Authors: Esposito CL, Autiero I, Di Ruscio A

Article Snippet:PrePrint: Bio-Layer Interferometry measurements were performed using a BLItz system and AR2G tips (Amine Reactive biosensors of 2nd Generation) (ForteBio Inc).Bio-Layer Interferometry measurements were performed using a BLItz system and AR2G tips (Amine Reactive biosensors of 2nd Generation) (ForteBio Inc).. After pre-hydration for 10 min in PBS buffer, the AR2G tips were efficiently functionalized with DNMT1 (Active Motif), DNMT3A (Abcam), DNMT3B (Abcam) and KAT 5 (KAT5-1350H; Creative BioMart) proteins following the manufacturer’s instructions.. The AR2G tips enable the coupling of proteins to carboxylate groups on the biosensor surface via accessible amine groups.The AR2G tips enable the coupling of proteins to carboxylate groups on the biosensor surface via accessible amine groups.

( a-c ) Binding measured by bio-layer interferometry of Ce-49 sh ( a ), Ce-10-2 sh ( b ) and DNMT1 bait ( c ) to DNMT3A ( left panels ), DNMT3B ( middle panels ) and KAT5 protein ( right panels ) immobilized on separate biosensors. Aptamers were tested at the reported concentrations. ( d ) ELONA assay of indicated biotinylated aptamers on plates left uncoated (-) or coated with HSA purified protein.