Active Recombinant Human KDR Protein, His & Avi-tagged, Biotinylated

• Cat.No.: KDR-6967H
• Product Overview: Biotinylated Recombinant Human KDR Protein (Ala 20 - Glu 764) (AAI31823), fused with an Avi tag at the C-terminus, followed by a polyhistidine tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: His & Avi
• Predicted N Terminal: Ala 20
• Form: Lyophilized from sterile PBS, pH7.4, 10% trehalose.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized Human VEGF, Tag Free at 2μg/mL (100 μL/well) can bind Biotinylated Human VEGF R2, His Tag with a linear range of 0.15-2.5 μg/mL.
• Molecular Mass: Recombinant Human KDR Protein has a calculated MW of 86 kDa. The protein migrates as 120-130 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation.
• Protein length: Ala 20 - Glu 764
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: KDR kinase insert domain receptor (a type III receptor tyrosine kinase) [ Homo sapiens ]
• Official Symbol: KDR
• Synonyms: KDR; kinase insert domain receptor (a type III receptor tyrosine kinase); vascular endothelial growth factor receptor 2; CD309; FLK1; VEGFR; VEGFR2; soluble VEGFR2; fetal liver kinase 1; fetal liver kinase-1; protein-tyrosine kinase receptor Flk-1; tyrosine kinase growth factor receptor
• Gene ID: 3791
• mRNA Refseq: NM_002253
• Protein Refseq: NP_002244
• MIM: 191306
• UniProt ID: P35968
• Chromosome Location: 4q11-q12
• Pathway: Angiogenesis, organism-specific biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Endocytosis, organism-specific biosystem; Endocytosis, conserved biosystem; Focal Adhesion, organism-specific biosystem; Focal adhesion, organism-specific biosystem
• Function: ATP binding; Hsp90 protein binding; growth factor binding; integrin binding; nucleotide binding; protein binding; protein tyrosine kinase activity; receptor activity; receptor signaling protein tyrosine kinase activity; transmembrane receptor protein tyrosine kinase activity; vascular endothelial growth factor-activated receptor activity