Active Recombinant Human LAG3 protein, Fc/Avi-tagged, Biotinylated
Cat.No. : | LAG3-051H |
Product Overview : | Biotinylated Recombinant Human LAG3(Leu23-Leu450) protein, fused to Fc/Avi tag at the C-terminus, was expressed in CHO cells . |
- Specification
- Gene Information
- Related Products
Description : | LAG-3 (Lymphocyte activation gene-3), designated CD223, is a 70 kDa type I transmembrane protein that is a member of the immunoglobulin superfamily (IgSF) (1, 2). LAG-3 shares approximately 20% amino acid sequence homology with CD4, but has similar structure and binds to MHC class II with higher affinity, providing negative regulation of T cell receptor signaling (1, 2). Human LAG-3 cDNA encodes 525 amino acids (aa) that include a 28 aa signal sequence, a 422 aa extracellular domain (ECD) with four Ig-like domains, a transmembrane region and a highly charged cytoplasmic region. Within the ECD, human LAG-3 shares 70%, 67%, 76%, and 73% aa sequence identity with mouse, rat, porcine, and bovine LAG-3, respectively. LAG-3 is expressed on activated CD4+ and CD8+ T cells, NK cells, and plasmacytoid dendritic cells (pDC), but not on resting T cells (1-3). LAG-3 on activated CD4+CD25+ Treg cells plays a role in their suppressive activity (4). LAG-3 limits the expansion of activated T cells and pDC in response to selected stimuli (3-5). A soluble 54 kDa form, sLAG-3, can be shed by metalloproteinases ADAM10 and TACE/ADAM17 (6, 7). While monomeric sLAG-3 itself may be inactive, shedding allows for normal T cell activation by removing negative regulation (7). Binding of a homodimerized sLAG-3/Ig fusion protein to MHC class II molecules induces maturation of immature DC, and secretion of cytokines such as IFN-gamma and TNF-alpha by type 1 cytotoxic CD8+ T cells and NK cells (8, 9). sLAG-3/Ig has been used as a potential adjuvant to stimulate a cytotoxic anti-cancer immune response (9, 10). In mice, deletion of LAG-3 and another negative regulator, PD-1, facilitates anti-cancer response but also blocks self-tolerance and increases susceptibility to autoimmune diseases (11, 12). In humans, antibody-mediated down‑regulation of LAG-3 and PD-1 allows more effective control of chronic malaria, while in NOD (non‑obese diabetic) mice, deletion of LAG-3 alone accelerates diabetes (12-14). |
Source : | CHO cells |
Species : | Human |
Tag : | Fc/Avi |
Predicted N Terminal : | Leu23 |
Form : | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
Bio-activity : | Measured by its binding ability in a functional ELISA. When Recombinant HumanFGL1 His-tag is immobilized at 1 μg/mL (100 μL/well), the concentration of Recombinant Human LAG3 Fc Chimera Avi-tag that produces 50% of the optimal binding response is 0.1-0.6 μg/mL. |
Molecular Mass : | 81-92 kDa, under reducing conditions |
Protein length : | Leu23-Leu450 |
Endotoxin : | <0.10 EU per 1 μg of the protein by the LAL method. |
Purity : | >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. |
Applications : | Bioactivity |
Storage : | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 °C as supplied.1 month, 2 to 8 °C under sterile conditions after reconstitution.3 months, -20 to -70 °C under sterile conditions after reconstitution. |
Reconstitution : | Reconstitute at 500 μg/mL in PBS. |
Gene Name : | LAG3 lymphocyte-activation gene 3 [ Homo sapiens ] |
Official Symbol : | LAG3 |
Synonyms : | LAG3; lymphocyte-activation gene 3; lymphocyte activation gene 3 protein; CD223; |
Gene ID : | 3902 |
mRNA Refseq : | NM_002286 |
Protein Refseq : | NP_002277 |
MIM : | 153337 |
UniProt ID : | P18627 |
Products Types
◆ Lysates | ||
LAG3-941RCL | Recombinant Rat LAG3 cell lysate | +Inquiry |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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