Active Recombinant Human LAMA1 protein, Fc-tagged

• Cat.No.: LAMA1-0345H
• Product Overview: Recombinant Human LAMA1 protein(Phe1878-Gln2700(Lamininlpha1E8)&Asp1714–Leu1786(Lamininbeta1E8)&Asp1528–Pro1609(Laminingamma1E8)), fused with Fc tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: Fc
• Form: Lyophilized from 0.22 μm filtered solution in 51 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 with trehalose as protectant.
• Bio-activity: Immobilized Human Laminin 111 E8, Fc Tag at 5 μg/mL (100 μL/well) can bind Human ITGA7X2B&ITGB1 Heterodimer Protein, His Tag&Tag Free with a linear range of 0.02-0.313 μg/mL.Immobilized Human ITGA6&ITGB1 Heterodimer Protein, His Tag&Tag Free at 5 μg/mL (100 μL/well) can bind Human Laminin 111 E8, Fc Tag with a linear range of 0.5-31 ng/mL.Immobilized Human ITGA7X2B&ITGB1 Heterodimer Protein, His Tag&Tag Free at 5 μg/mL (100 μL/well) can bind Human Laminin 111 E8, Fc Tag with a linear range of 0.2-2 ng/mL.
• Molecular Mass: This protein carries a human IgG1 Fc tag at the N-terminusThe protein has a calculated MW of 92.2 kDa | 35 kDa | 35.6 kDa. The protein migrates as 70-80 kDa and 116-125 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Phe1878-Gln2700(Lamininlpha1E8)&Asp1714–Leu1786(Lamininbeta1E8)&Asp1528–Pro1609(Laminingamma1E8)
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: LAMA1 laminin, alpha 1 [ Homo sapiens ]
• Official Symbol: LAMA1
• Synonyms: LAMA1; laminin, alpha 1; LAMA; laminin subunit alpha-1; S-LAM alpha; laminin A chain; S-laminin subunit alpha; laminin-3 subunit alpha; S-LAM-alpha
• Gene ID: 284217
• mRNA Refseq: NM_005559
• Protein Refseq: NP_005550
• MIM: 150320
• UniProt ID: P25391