Active Recombinant Human Leptin Antagonist Triple Mutant, PEG-tagged

• Cat.No.: LEP-260H
• Product Overview: Pegylated Leptin Antagonist Triple Mutant Human Recombinant is a single non-glycosilated polypeptide chain containing 146 amino and additional Ala at N-terminus acids and having a molecular weight of 35.6kDa, Leptin was mutated, resulting in L39A/D40A/F41A. However due to enlarged hydrodymanic volume it runs on the SDS-PAGE as 48 kDa protein and in gel-filtration on Superdex 200 as over 200 kDa protein. Leptin Antagonist Triple Mutant Human Recombinant is Mono-Pegylated with 20kDa PEG and was purified by proprietary chromatographic techniques.

Specification

• Species: Human
• Source: Human
• Tag: Non
• Form: The protein was lyophilized from a concentrated (0.65mg/ml) solution with 0.003mM NaHCO3.
• Purity: Greater than 98.0% as determined by: (a) Gel filtration analysis. (b) Analysis by SDS-PAGE.
• Bio-activity: Capable of inhibiting leptin-induced proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor. Its in vitro activity is 6-8 fold lower than the non-pegylated antagonist but in vivo it has profound weight gain effect in mice (as compared to the non-pegylated antagonist), resulting mainly from increased food intake.
• Physical Appearance: White lyophilized (freeze-dried) powder.
• Solubility: It is recommended to reconstitute the lyophilized Leptin Antagonist Triple Mutant pegylated Human Recombinant in sterile 0.4% NaHCO3 adjusted to pH 8-9, not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
• Protein content: Protein quantitation was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.88 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
• Storage: Lyophilized Pegylated Leptin Antagonist Triple Mutant Human Recombinant although stable at room temperature for several weeks, should be C. Upon reconstitution at°stored desiccated below -18 > 0.1 mg/ml and up to C or even room 2mM and filter sterilization Leptin mutant can be stored at 4 temperature for several weeks making it suitable for long term infusion studies using osmotic pumps. At lower concentration addition of a carrier protein (0.1% HSA or BSA) is suggested. Please prevent freeze-thaw cycles.

Gene Information

• Gene Name: LEP leptin [ Homo sapiens ]
• Official Symbol: LEP
• Synonyms: LEP; leptin; OB; OBS; FLJ94114; obesity factor; obese, mouse, homolog of; leptin (murine obesity homolog); leptin (obesity homolog, mouse); Obese protein; Obesity factor
• Gene ID: 3952
• mRNA Refseq: NM_000230
• Protein Refseq: NP_000221
• MIM: 164160
• UniProt ID: P41159
• Chromosome Location: 7q31.3
• Pathway: Adipocytokine signaling pathway; Cytokine-cytokine receptor interaction; Jak-STAT signaling pathway; Neuroactive ligand-receptor interaction
• Function: growth factor activity; hormone activity; peptide hormone receptor binding; protein binding