Active Recombinant Human Leucine Rich Repeat Transmembrane Neuronal 3, His-tagged

• Cat.No.: LRRTM3-5683H
• Product Overview: Recombinant Human LRRTM3protein was expressed in Murine myeloma cell line with a C-terminal 6-Histag. Glu31-Lys419.

Specification

• Species: Human
• Source: Mammalian Cells
• Tag: His
• Protein Length: 31-419 a.a.
• Description: Human LRRTM3 is a 63kDa type I transmembrane protein, and one of four members of the LRRTM familyof proteins within the leucine-rich repeat superfamily. There are twoisoforms of LRRTM3. Isoform 1 is synthesized as a precursor with a 30 aminoacid signal sequence, a 389 aa extracellular region, a 21 aa transmembraneregion, and a 141 aa cytoplasmic region. In isoform 2, aa 513 is changed fromisoleucine to valine, and aa 514 to 581 are missing, producing a cytoplasmicregion of only 74 aa.
• Form: Lyophilized from a0.2 μm filtered solution in PBS.
• N-terminalSequence: Glu31
• Molecular Weight: 45.2 kDa
• Activity: Measuredby its ability to enhance neurite outgrowth of E16-E18 rat embryonic corticalneurons. Able to significantly enhance neurite outgrowth when immobilized asa 3 μL droplet containing 60-250 ng on a nitrocellulose-coated microplate.
• Endotoxin Level: <1.0 EUper 1 μg of the protein by the LAL method.
• SDS-PAGE: 50-60kDa, reducing conditions.
• Purity: >90%,by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution: 100μg/mL in sterile PBS
• Storage: Use amanual defrost freezer and avoid repeated freeze-thaw cycles. 12 months fromdate of receipt, -20 to -70°C as supplied. 1 month, 2 to 8°C under sterileconditions after reconstitution. 3 months, -20 to -70°C under sterileconditions after reconstitution.

Gene Information

• Gene Name: LRRTM3 leucine rich repeattransmembrane neuronal 3 [ Homo sapiens ]
• Official Symbol: LRRTM3
• Synonyms: LRRTM3; leucine richrepeat transmembrane neuronal 3; MGC131810; leucine-rich repeat transmembraneneuronal protein 3; UNQ803/PRO1693; OTTHUMP00000019682; OTTHUMP00000019683
• Gene ID: 347731
• mRNA Refseq: NM_178011
• Protein Refseq: NP_821079
• MIM: 610869
• UniProt ID: Q86VH5
• Chromosome Location: 10q22.1