Active Recombinant Human LGALS9 protein

• Cat.No.: LGALS9-443H
• Product Overview: Recombinant Human LGALS9(Ala2-Thr323) was expressed in E. coli.

Specification

• Source: E. coli
• Species: Human
• Predicted N Terminal: Ala2
• Form: Lyophilized from a 0.2 μm filtered solution in MOPS, NaCl, EDTA, DTT and Trehalose.
• Bio-activity: Measured by its ability to induce apoptosis of Jurkat human acute T cell leukemia cells. Lu, L.H. et al. (2007) J. Biochem. 141:157. The ED50 for this effect is typically 1-5 μg/mL. Measured by its ability to agglutinate human red blood cells. Hadari, Y.R. et al. (2000) J. Cell Sci. 113:2385. The ED50 for this effect is typically 2.5-12.5 µg/mL.
• Molecular Mass: Predicted Molecular Mass: 35.8 kDa;SDS-PAGE: 34 kDa, reducing conditions.
• Endotoxin: <1.0 EU per 1 μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 centigrade as supplied.1 month, 2 to 8 centigrade under sterile conditions after reconstitution.3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Reconstitution: Reconstitute at 100 μg/mL in water.

Gene Information

• Gene Name: LGALS9 lectin, galactoside-binding, soluble, 9 [ Homo sapiens ]
• Official Symbol: LGALS9
• Synonyms: LGALS9; lectin, galactoside-binding, soluble, 9; galectin-9; galectin 9; LGALS9A; gal-9; ecalectin; tumor antigen HOM-HD-21; urate transporter/channel protein; HUAT; MGC117375; MGC125973; MGC125974
• Gene ID: 3965
• mRNA Refseq: NM_002308
• Protein Refseq: NP_002299
• MIM: 601879
• UniProt ID: O00182
• Chromosome Location: 17q11.2
• Pathway: 1. Germinal center kinase-like kinase (GLK/MAP4K3) expression is increased in adult-onset Still's disease and may act as an activity marker. Chen DY, et al. BMC Med, 2012 Aug 6. 2. MAP4K3 enhances the expression of the BH3-only protein BID. Lam D, et al. Cell Cycle, 2009 Oct 15. 3. Activation of the c-Jun N-terminal kinase pathway by a novel protein kinase related to human germinal center kinase. Diener K, et al. Proc Natl Acad Sci U S A, 1997 Sep 2.
• Function: galactose binding; signal transducer activity; sugar binding; sugar binding