Active Recombinant Human MAP1LC3B

Cat.No. : MAP1LC3B-194H
Product Overview : Recombinant human MAP1LC3B (amino acid residues 1-120) was expressed in E.coli.
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Description : Three classes of enzymes are involved in autophagy; E1-like activating enzymes (E1s), E2-like conjugating enzymes (E2s) and ubiquitin-like proteins (ubls). Microtubule Associated Protein 1, Light Chain 3 beta, MAP1LC3b or LC3b is a member of the Ubl family and cloning of the human gene was first described by He et al. (2003). The C-terminal of LC3b is cleaved by ATG4B C-terminal to Gly120 in vitro and this has been shown to be required for the formation of intermediates with the ubiquitin-like activating enzyme ATG7 (Tanida et al. 2004).
Source : E. coli
Species : Human
Form : 50 mM HEPES pH 7.5, 150 mM sodium chloride, 2 mM dithiothreitol, 10% glycerol
Bio-activity : E1 Thioester LC3b Loading Assay: The activity of LC3b was validated by loading LC3b onto the active cysteine of His-ATG7. Incubation of the His-ATG7 enzyme in the presence of LC3b and ATP at 30?C was compared at two time points, T0 and T10 minutes. Sensitivity of the LC3b/His-ATG7 thioester bond to the reducing agent DTT was confirmed.
Molecular Mass : ~14.84 kDa
Purity : >98% by SDS-PAGE
Storage : 12 months at -70°C. Avoid multiple freeze/thaw cycles.
Concentration : 1 mg/ml
Gene Name : MAP1LC3B microtubule-associated protein 1 light chain 3 beta [ Homo sapiens ]
Official Symbol : MAP1LC3B
Synonyms : MAP1LC3B; microtubule-associated protein 1 light chain 3 beta; microtubule-associated proteins 1A/1B light chain 3B; ATG8F; MAP1A/MAP1B LC3 B; MAP1A/MAP1B light chain 3 B; MAP1 light chain 3-like protein 2; autophagy-related ubiquitin-like modifier LC3 B; LC3B; MAP1LC3B-a; MAP1A/1BLC3;
Gene ID : 81631
mRNA Refseq : NM_022818
Protein Refseq : NP_073729
MIM : 609604
UniProt ID : Q9GZQ8
Chromosome Location : 16q24.2
Pathway : Senescence and Autophagy, organism-specific biosystem;
Function : protein binding;

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