Active Recombinant Human MICB protein, His-tagged

• Cat.No.: MICB-808H
• Product Overview: Active Recombinant Human MICB protein(NP_005922.2) (Met 1-Gly 298) was expressed in HEK293 with a C-terminal polyhistidine tag.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 1-298 aa
• Form: Lyophilized from sterile PBS, pH 7.4.
• Bio-activity: Immobilized human MICB-His at 10 μg/ml (100 μl/well) can bind S4-Fc3L3-NKG2D, The EC50 of S4-Fc3L3-NKG2D is 0.52-1.2 μg/ml.
• Molecular Mass: The recombinant human MICB consists of 287 amino acids and has a predicted molecular mass of 33 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh MICB is approximately 45-50 kDa due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 98 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: MICB MHC class I polypeptide-related sequence B [ Homo sapiens ]
• Official Symbol: MICB
• Synonyms: MICB; MHC class I polypeptide-related sequence B; PERB11.2; MIC-B; MHC class I mic-B antigen; stress inducible class I homolog; MHC class I chain-related protein B; MHC class I-like molecule PERB11.2-IMX
• Gene ID: 4277
• mRNA Refseq: NM_005931
• Protein Refseq: NP_005922
• MIM: 602436
• UniProt ID: Q29980