Active Recombinant Human NCAM1

• Cat.No.: NCAM1-660H
• Product Overview: Recombinant Human NCAM1 (Accession # NP_001070150) was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Species: Human
• Source: Mammalian Cells
• Tag: Non
• Predicted N Terminal: Leu20
• Form: Lyophilized from a 0.2 µm filtered solution in Tris and NaCl.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of Neuro-2A mouse neuroblastoma cells.When 5 x 10^4 cells/well are added to recombinant human NCAM-1 coated plates, cell adhesion is enhanced in a dose dependent manner after 1 hour incubation at 37 °C. The ED50 for this effect is typically 0.5 - 3 µg/mL. Optimal dilutions should be determined by each laboratory for each application.
• Molecular Mass: Recombinant Human NCAM1 has a calculated MW of 76.5 kDa. In SDS-PAGE migrates as 120 kDa, reducing conditions.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: NCAM1 neural cell adhesion molecule 1 [ Homo sapiens ]
• Official Symbol: NCAM1
• Synonyms: NCAM1; neural cell adhesion molecule 1; CD56; NCAM; neural cell adhesion molecule, NCAM; antigen recognized by monoclonal 5.1H11; MSK39
• Gene ID: 4684
• mRNA Refseq: NM_000615
• Protein Refseq: NP_000606
• MIM: 116930
• UniProt ID: P13591
• Chromosome Location: 11q23.2