Active Recombinant Human NEDD8

• Cat.No.: NEDD8-160H
• Product Overview: Recombinant human NEDD8 (amino acid residues 1-76) was expressed in E. coli.

Specification

• Description: Neural Precursor Cell Expressed, Developmentally Downregulated 8 (NEDD8) is a member of the ubiquitin like modifiers and the human gene was first described by (Kamitani, et al., 1997). The heterodimeric E1, APP-BP1/UBA3 forms a complex in vitro and a thioester linkage with NEDD8 (Osaka et al., 1998). NEDD8 has also been co-crystallised with APP-BP1 and ATP (Walden et al., 2003).
• Source: E. coli
• Species: Human
• Form: 50 mM HEPES pH 7.5, 150 mM sodium chloride, 2 mM dithiothreitol, 10% glycerol
• Bio-activity: E2 thioester NEDD8 loading assay. The activity of NEDD8 was validated by loading E1 APP-BP1/Uba3 activated NEDD8 onto the active cysteine of the His-UBE2M E2 enzyme via a transthiolation reaction. Incubation of the APP-BP1/Uba3 and His-UBE2M enzymes in the presence of NEDD8 and ATP at 30?C was compared at two time points, T0 and T10 minutes. Sensitivity of the NEDD8/His-UBE2M thioester bond to the reducing agent DTT was confirmed.
• Molecular Mass: ~8.6kDa
• Purity: >98% by SDS-PAGE
• Storage: 12 months at -70°C. Avoid multiple freeze/thaw cycles.
• Concentration: 1 mg/ml

Gene Information

• Gene Name: NEDD8 neural precursor cell expressed, developmentally down-regulated 8 [ Homo sapiens ]
• Official Symbol: NEDD8
• Synonyms: NEDD8; neural precursor cell expressed, developmentally down-regulated 8; Nedd 8; neddylin; ubiquitin-like protein Nedd8; NEDD-8; FLJ43224; MGC104393; MGC125896; MGC125897
• Gene ID: 4738
• mRNA Refseq: NM_006156
• Protein Refseq: NP_006147
• MIM: 603171
• UniProt ID: Q15843
• Chromosome Location: 14q11.2
• Pathway: p53 pathway, organism-specific biosystem
• Function: ubiquitin protein ligase binding