Active Recombinant Human NELL1 Protein, His-tagged

• Cat.No.: NELL1-01H
• Product Overview: Recombinant Human NELL1 Protein (Arg17-Asn810), with a C-terminal 6-His tag was expressed in Chinese Hamster Ovary cell line.

Specification

• Species: Human
• Source: CHO
• Tag: His
• Protein Length: 17-810 a.a.
• Description: This gene encodes a cytoplasmic protein that contains epidermal growth factor (EGF)-like repeats. The encoded heterotrimeric protein may be involved in cell growth regulation and differentiation. A similar protein in rodents is involved in craniosynostosis. Alternative splicing results in multiple transcript variants.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is 1‑4 μg/mL.
• Molecular Mass: 88.6 kDa & 88.1 kDa
• N-terminal Sequence Analysis: Arg17 (33%) & Phe22 (66%)
• Endotoxin: <0.01 EU/μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE with silver staining
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Reconstitution: Reconstitute at 300 μg/mL in PBS.
• Shipping: The product is shipped at ambient temperature.

Gene Information

• Gene Name: NELL1 neural EGFL like 1 [ Homo sapiens (human) ]
• Official Symbol: NELL1
• Synonyms: NELL1; neural EGFL like 1; NRP1; IDH3GL; protein kinase C-binding protein NELL1; nel-related protein 1; neural epidermal growth factor-like 1
• Gene ID: 4745
• mRNA Refseq: NM_006157
• Protein Refseq: NP_006148
• MIM: 602319
• UniProt ID: Q92832