Active Recombinant Human NNMT Protein, His-tagged, Bioactivity Validated

• Cat.No.: NNMT-234H
• Product Overview: Recombinant Human NNMT(1-264aa) fused with His tag at N-terminal was expressed in E. coli. Bioactivity Validated.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-264aa
• Description: NNMT(Nicotinamide N-methyltransferase) belongs to the family of transferases, specifically those transferring one-carbon group methyltransferases. It is predominantly expressed in the liver, and a lower expression is seen in the kidney, lung, skeletal muscle, placenta and heart. NNMT catalyzes the N-methylation of nicotinamide and other pyridines to form pyridinium ions. This activity is important for biotransformation of many drugs and xenobiotic compounds.
• Form: Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol
• Bio-activity: Specific activity is > 100 nmol/min/mg, and is defined as the amount of enzyme that transfer 1.0 nmole of methyl group per minute at 37C.
• Molecular Mass: 37.7 kDa (284aa), confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MESGFTSKDT YLSHFNPRDY LEKYYKFGSR HSAESQILKH LLKNLFKIFC LDGVKGDLLI DIGSGPTIYQ
  LLSACESFKE IVVTDYSDQN LQELEKWLKK EPEAFDWSPV VTYVCDLEGN RVKGPEKEEK LRQAVKQVLK CDVTQSQPLG AVPLPPADCV
  LSTLCLDAAC PDLPTYCRAL RNLGSLLKPG GFLVIMDALK SSYYMIGEQK FSSLPLGREA VEAAVKEAGY TIEWFEVISQ SYSSTMANNE
  GLFSLVARKL SRPL
• Purity: > 95% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 1mg/ml (determined by Absorbance at 280nm)

Gene Information

• Gene Name: NNMT nicotinamide N-methyltransferase [ Homo sapiens ]
• Official Symbol: NNMT
• Synonyms: NNMT; nicotinamide N-methyltransferase
• Gene ID: 4837
• mRNA Refseq: NM_006169
• Protein Refseq: NP_006160
• MIM: 600008
• UniProt ID: P40261