Active Recombinant Human OSM protein, His-tagged

• Cat.No.: OSM-6998H
• Product Overview: Recombinant Human OSM protein(P13725)(Met1-Arg221), fused with His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Met1-Arg221
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: 1. Measured by its binding ability in a functional ELISA. Immobilized human OSM-His at 10 μg/ml (100 μl/well) can bind biotinylated human LIFR-His with a linear range of 0.031-0.5 μg/ml. 2. Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.5-2.5 ng/ml.
• Molecular Mass: The recombinant human OSM consists of 207 amino acids and has a predicted molecular mass of 23.6 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhOSM is approximately 32-35 kDa due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: OSM oncostatin M [ Homo sapiens ]
• Official Symbol: OSM
• Synonyms: OSM; oncostatin M; oncostatin-M; MGC20461
• Gene ID: 5008
• mRNA Refseq: NM_020530
• Protein Refseq: NP_065391
• MIM: 165095
• UniProt ID: P13725