Active Recombinant Human OTUD1, His-tagged

Cat.No. : OTUD1-128H
Product Overview : Recombinant Human OTUD1 CD(270-481), fused with N-terminal 6His, was expressed in E.coli.
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Description : OTUD1 is a cysteine protease and is a member of the OTU superfamily of proteins (Balakirev et al., 2003). Cloning of the human gene was first described by Kayagaki et al. (2007).
Source : E. coli
Species : Human
Tag : His
Form : 50 mM HEPES pH 7.5, 150 mM sodium chloride, 2 mM dithiothreitol, 10% glycerol
Bio-activity : Deubiquitylase Enzyme Assay: The activity of His-OTUD1 was validated by determining the increase in fluorescence measured as a result of the enzyme catalysed cleavage of the fluorogenic substrate Ubiquitin-Rhodamine110-Glycine generating Ubiquitin and Rhodamine110-Glycine. Incubation of the substrate in the presence or absence of His-OTUD1 was compared confirming the deubiquitylating activity of His-OTUD1.
Molecular Mass : ~30kDa
Storage : 12 months at -70℃; aliquot as required.
Concentration : 0.5 mg/ml
Gene Name : OTUD1 OTU domain containing 1 [ Homo sapiens ]
Official Symbol : OTUD1
Synonyms : DUBA7; OTDC1
Gene ID : 220213
mRNA Refseq : NM_001145373.2
Protein Refseq : NP_001138845.1
MIM : 612022
UniProt ID : Q5VV17
Chromosome Location : 10p12.2

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.


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