Active Recombinant Human OTUD1, His-tagged

• Cat.No.: OTUD1-128H
• Product Overview: Recombinant Human OTUD1 CD(270-481), fused with N-terminal 6His, was expressed in E.coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 270-481 a.a.
• Description: OTUD1 is a cysteine protease and is a member of the OTU superfamily of proteins (Balakirev et al., 2003). Cloning of the human gene was first described by Kayagaki et al. (2007).
• Form: 50 mM HEPES pH 7.5, 150 mM sodium chloride, 2 mM dithiothreitol, 10% glycerol
• Bio-activity: Deubiquitylase Enzyme Assay: The activity of His-OTUD1 was validated by determining the increase in fluorescence measured as a result of the enzyme catalysed cleavage of the fluorogenic substrate Ubiquitin-Rhodamine110-Glycine generating Ubiquitin and Rhodamine110-Glycine. Incubation of the substrate in the presence or absence of His-OTUD1 was compared confirming the deubiquitylating activity of His-OTUD1.
• Molecular Mass: ~30kDa
• Storage: 12 months at -70℃; aliquot as required.
• Concentration: 0.5 mg/ml

Gene Information

• Gene Name: OTUD1 OTU domain containing 1 [ Homo sapiens ]
• Official Symbol: OTUD1
• Synonyms: DUBA7; OTDC1
• Gene ID: 220213
• mRNA Refseq: NM_001145373.2
• Protein Refseq: NP_001138845.1
• MIM: 612022
• UniProt ID: Q5VV17
• Chromosome Location: 10p12.2