Active Recombinant Human PDGFB
Cat.No. : | PDGFB-146H |
Product Overview : | Recombinant Human platelet-derived growth factor was expressed in modifiedhuman 293 cells. |
- Specification
- Gene Information
- Related Products
Cat. No. : | PDGFB-146H |
Description : | The platelet-derived growth factor (PDGF) family consists of four different polypeptide chains; PDGF-A, PDGF-B, PDGF-C and PDGF-D, sharing approximately 25% sequence homology. The biological effects of PDGF-B are exerted via two receptor tyrosine kinases, PDGFRA and PDGFRB. PDGF-B is expressed predominantly in platelets and the heart, but has been detected in the majority of human organs. PDGF-B is a potent mitogen for mesenchymal cells and plays a critical role in wound healing and tumor development. PDGF-B is an important growth factor contributing to angiogenesis and possibly lymphatic metastasis due to lymphangiogenesis. Additionally, PDGF-B plays a crucial role in wound healing. Studies have also suggested that PDGF-B is involved in the CNS including having a neuroprotective or neurotrophic effect in the developing or adult CNS, promoting angiogenic responses to injury in the CNS and remyelination.. |
Source : | human 293 cells. |
Molecular Mass : | Under reducing conditions PDGF-B migrates as a broad band between 15.2 and 16.1 kDa on SDS-PAGE due to post-translational modifications, in particular glycosylation. This compares with unmodified PDGF-B polypeptide that has a predicted monomeric molecular mass of 12.29 kDa. |
PI : | PDGF-B has a predicted pI of 9.38. |
% Carbohydrate : | purified PDGF-B consists of 30-45% carbohydrate by weight. |
Glycosylation : | PDGF-B contains N-linked oligosaccharides and a predicted O-linked Mannose. |
Purity : | >95%, as determined by SDS-PAGE, visualized by Coomassie Brilliant Blue. |
Formulation : | When reconstituted in 0.5 ml sterile phosphate-buffered saline, the solution will contain 1% human serum albumin (HSA) and 10% trehalose. |
Reconstitution : | It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial. |
Storage : | Lyophilized products should be stored at 2 to 8°C. Following reconstitution short-term storage at 4°C is recommended, with longer-term storage in aliquots at -18 to -20°C. |
Activity : | The ED50of PDGF-B is typically 14-25 ng/mL as measured in a cell proliferation assay using the murine Balb/3T3 fibroblast cell line. |
Theoretical Sequence : | SLGSLTI AEPAMIAECK TRTEVFEISRRLI DRTNANFLVWPPC VEVQRCSGCCNNR NV QCRP TQVQLRPV QVRKI EIVRK KPIFKK ATVTLEDHLACKCETVAAARPV. |
Gene Name : | PDGFB platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog) [ Homo sapiens ] |
Synonyms : | platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog); SIS; SSV; PDGF2; c-sis; FLJ12858; PDGFB; platelet-derived growth factor beta; becaplermin; PDGF, B chain; platelet-derived growth factor 2; platelet-derived growth factor, B chain;Platelet-derived growth factor, beta polypeptide (oncogene SIS); oncogene SIS; Becaplermin; PDGF-2; PDGF subunit B; Platelet-derived growth factor beta polypeptide; Platelet-derived growth factor B chain |
Gene ID : | 5155 |
mRNA Refseq : | NM_002608 |
Protein Refseq : | NP_002599 |
MIM : | 190040 |
UniProt ID : | P01127 |
Chromosome Location : | 22q13.1 |
Pathway : | Cytokine-cytokine receptor interaction; Focal adhesion; Gap junction; Glioma; MAPK signaling pathway; Melanoma; Pathways in cancer; Prostate cancer; Regulation of actin cytoskeleton; Renal cell carcinoma; Hemostasis; Signaling by PDGF |
Function : | cell surface binding; collagen binding; growth factor activity; platelet-derived growth factor binding platelet-derived growth factor receptor binding; protein heterodimerization activity; protein homodimerization activity |
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◆ Lysates | ||
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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