Active Recombinant Human PGA4 protein, His-tagged

• Cat.No.: PGA4-1110H
• Product Overview: Active Recombinant Human PGA4 protein(P00790) (Met 1-Ala 388) was expressed in HEK293, fused with a polyhistidine tag at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 1-388 aa
• Form: Lyophilized from sterile PBS, pH 7.4.
• Bio-activity: Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2. The specific activity is > 10,000 pmoles/min/μg.
• Molecular Mass: The secreted recombinant human PGA4 consists of 384 amino acids and has a predicted molecular mass of 41.7 kDa. It migrates as an approximately 45 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 97 % as determined by SDS-PAGE.
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.