Active Recombinant Human PGK2 Protein, His-tagged

• Cat.No.: PGK2-3982H
• Product Overview: Recombinant human PGK2 protein (1-417aa), fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-417
• Description: This gene is intronless, arose via retrotransposition of the phosphoglycerate kinase 1 gene, and is expressed specifically in the testis. Initially assumed to be a pseudogene, the encoded protein is actually a functional phosphoglycerate kinase that catalyzes the reversible conversion of 1,3-bisphosphoglycerate to 3-phosphoglycerate, during the Embden-Meyerhof-Parnas pathway of glycolysis, in the later stages of spermatogenesis.
• Form: Liquid
• Bio-activity: > 500 unit/mg. One unit will convert 1 umole of 1,3-Bisphosphoglycerate to 3-PGA per minute at pH 8.0 at 37 centigrade.
• Molecular Mass: 46.9 kDa (437aa) confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHHSSGLVPRGSHMSLSKKLTLDKLDVRGKRVIMRVDFNVPMKKNQITNNQRIKASIPSIKYCLDNGAKAVVLMSHLGRPDGVPMPDKYSLAPVAVELKSLLGKDVLFLKDCVGAEVEKACANPAPGSVILLENLRFHVEEEGKGQDPSGKKIKAEPDKIEAFRASLSKLGDVYVNDAFGTAHRAHSSMVGVNLPHKASGFLMKKELDYFAKALENPVRPFLAILGGAKVADKIQLIKNMLDKVNEMIIGGGMAYTFLKVLNNMEIGASLFDEEGAKIVKDIMAKAQKNGVRITFPVDFVTGDKFDENAQVGKATVASGISPGWMGLDCGPESNKNHAQVVAQARLIVWNGPLGVFEWDAFAKGTKALMDEIVKATSKGCITVIGGGDTATCCAKWNTEDKVSHVSTGGGASLELLEGKILPGVEALSNM
• Purity: > 95% by SDS-PAGE
• Applications: Enzyme Activity, SDS-PAGE
• Storage: Can be stored at 2 to 8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/mL (determined by Bradford assay)
• Storage Buffer: 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 1 mM DTT, 0.1 M NaCl

Gene Information

• Gene Name: PGK2 phosphoglycerate kinase 2 [ Homo sapiens (human) ]
• Official Symbol: PGK2
• Synonyms: PGK2; phosphoglycerate kinase 2; PGKB; PGKPS; HEL-S-272; dJ417L20.2; phosphoglycerate kinase 2; epididymis secretory protein Li 272; phosphoglycerate kinase autosomal pseudogene; phosphoglycerate kinase, testis specific; testicular tissue protein Li 139; EC 2.7.2.3
• Gene ID: 5232
• mRNA Refseq: NM_138733
• Protein Refseq: NP_620061
• MIM: 172270
• UniProt ID: P07205