Active Recombinant Human PHEX, His-tagged

• Cat.No.: PHEX-179H
• Product Overview: Recombinant Human PHEX (Gln42-Trp749), fused with an N-terminal 6-His tag, was expressed in Sf 21 (baculovirus).

Specification

• Description: The protein encoded by this gene is a transmembrane endopeptidase that belongs to the type II integral membrane zinc-dependent endopeptidase family. The protein is thought to be involved in bone and dentin mineralization and renal phosphate reabsorption. Mutations in this gene cause X-linked hypophosphatemic rickets. Alternative splicing results in multiple transcript variants.
• Source: Sf 21 (baculovirus)
• Species: Human
• Tag: His
• Predicted N Terminal: His & Leu47
• Form: Supplied as a 0.2 μm filtered solution in Tris and NaCl.
• Bio-activity: Measured by its ability to cleave a fluorogenic peptide substrate, Mca-YVADAPK. The specific activity is >125 pmol/min/μg.
• Molecular Mass: Predicted Molecular Mass: 83 kDaSDS-PAGE: 68-85 kDa, reducing conditions
• Endotoxin: < 1.0 eu per 1 μg of the protein by the lal method
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.
• Storage: Avoid repeated freeze-thaw cycles.6 months from date of receipt, -20 to -70 °C as supplied.3 months, -20 to -70 °C under sterile conditions after opening.

Gene Information

• Gene Name: PHEX phosphate regulating endopeptidase homolog, X-linked [ Homo sapiens (human) ]
• Official Symbol: PHEX
• Synonyms: PHEX; HYP; PEX; XLH; HPDR; HYP1; LXHR; HPDR1; phosphate regulating endopeptidase homolog, X-linked; phosphate-regulating neutral endopeptidase; metalloendopeptidase homolog PEX; X-linked hypophosphatemia protein; vitamin D-resistant hypophosphatemic rickets protein; phosphate regulating gene with homologies to endopeptidases on the X chromosome (hypophosphatemia, vitamin D resistant rickets)
• Gene ID: 5251
• mRNA Refseq: NM_000444
• Protein Refseq: NP_000435
• MIM: 300550
• UniProt ID: P78562
• Chromosome Location: Xp22.2-p22.1
• Function: aminopeptidase activity; metalloendopeptidase activity; zinc ion binding