Active Recombinant Human Platelet Endothelial Aggregation Receptor 1, His-tagged

• Cat.No.: PEAR1-498H
• Product Overview: Recombinant Human PEAR1protein was expressed in CHO with a C-terminal 6-His tag. Leu21-Ser754.

Specification

• Species: Human
• Source: CHO
• Tag: His
• Protein Length: 21-754 a.a.
• Description: PEAR1 is a 150 kDatype I transmembrane protein and member of the MEGF family of proteins. HumanPEAR1 is synthesized as a 1037 amino acid (aa) precursor that contains a 20aa signal sequence, a 735 aa extracellular domain (ECD), a 21 aatransmembrane region, and a 261 aa cytoplasmic region.
• Form: Lyophilized from a0.2 μm filtered solution in PBS.
• N-terminalSequence: Leu21
• Molecular Weight: 78.3 kDa
• Activity: Measuredby the ability of the immobilized protein to support the adhesion of thrombinactivated human platelets. When 5 x 106 cells per well are addedto rhPEAR1 coated plates (1 μg/mL, 100 μL/ well), approximately 50%-70% willadhere after 1 hour at 37°C.
• Endotoxin Level: <1.0 EUper 1 μg of the protein by the LAL method.
• SDS-PAGE: 120-130kDa, reducing conditions.
• Purity: >90%,by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution: 200 μg/ mLin PBS
• Storage: Use amanual defrost freezer and avoid repeated freeze-thaw cycles. 12 months fromdate of receipt, -20 to -70°C as supplied. 1 month, 2 to 8 °C under sterileconditions after reconstitution. 3 months, -20 to -70°C under sterileconditions after reconstitution.

Gene Information

• Gene Name: PEAR1 platelet endothelialaggregation receptor 1 [ Homo sapiens ]
• Official Symbol: PEAR1
• Synonyms: PEAR1; plateletendothelial aggregation receptor 1; JEDI; MEGF12; FLJ00193; Hpear1; multipleEGF-like-domains 12; multiple EGF-like domains protein 12; multiple epidermalgrowth factor-like domains protein 12; OTTHUMP00000038718
• Gene ID: 375033
• mRNA Refseq: NM_001080471
• Protein Refseq: NP_001073940
• MIM: 610278
• UniProt ID: Q5VY43
• Chromosome Location: 1q23.1