Active Recombinant Human POLR2K, GST-tagged

• Cat.No.: POLR2K-8510H
• Product Overview: Recombinant Human POLR2K,fused with GST tag,was expressed in E. coli.

Specification

• Description: RPB12 (also called RPB10 ) is shared between all three RNA polymerases and is homologous to the archaeal P subunit of RNA polymerase. Like RPB10, it is essential for yeast cell viability . RPB12 is also a zinc-binding protein with a canonical tetra-coordinating zinc motif but binds zinc in vitro much less efficiently than the RPB10 subunit. It has been shown to interact with both RPB1 and RPB2 in vitro and the human subunit can functionally substitute for its yeast counterpart.
• Source: E. coli
• Species: Human
• Tag: GST
• Form: 20 mM Tris-Cl, pH 7.9, 20% Glycerol, 100 mM KCl, 1 mM DTT and 0.2 mM EDTA
• Bio-activity: 1 unit equals 1 ng of purified protein. 100 units (ng) are sufficient for a protein-protein interaction assay.
• AA Sequence: MDTQKDVQPP KQQPMIYICG ECHTENEIKS RDPIRCRECG YRIMYKKRTK RLVVFDAR
• Purity: >95% as determined by SDS-PAGE
• Storage: -80 °C
• Concentration: 100 μg/ml
• Shipping: Dry Ice

Gene Information

• Gene Name: POLR2K polymerase (RNA) II (DNA directed) polypeptide K, 7.0kDa [ Homo sapiens ]
• Official Symbol: POLR2K
• Synonyms: POLR2K; polymerase (RNA) II (DNA directed) polypeptide K, 7.0kDa; RPB12; RPABC4; RPB7.0; hRPB7.0; hsRPB10a; RPB10alpha; ABC10-alpha; DNA-directed RNA polymerases I, II, and III subunit RPABC4; RNA polymerase II 7.0 kDa subunit; DNA-directed RNA polymerase II subunit K; RNA polymerases I, II, and III subunit ABC4; DNA directed RNA polymerases I, II, and III 7.0 kda polypeptide
• Gene ID: 5440
• mRNA Refseq: NM_005034
• Protein Refseq: NP_005025
• MIM: 606033
• UniProt ID: P53803
• Chromosome Location: 8q22.2
• Pathway: Abortive elongation of HIV-1 transcript in the absence of Tat, organism-specific biosystem; Cytosolic DNA-sensing pathway, organism-specific biosystem; DNA Repair, organism-specific biosystem
• Function: DNA binding; NA-directed RNA polymerase activity; metal ion binding