Active Recombinant human POR Protein, His-tagged

• Cat.No.: POR-01H
• Product Overview: Recombinant human POR (1-680aa), fused to His-tag at C-terminus, was Recombinant human POR protein expressed in insect cell and purified by using conventional chromatography techniques.

Specification

• Description: This gene encodes an endoplasmic reticulum membrane oxidoreductase that is essential for multiple metabolic processes, including reactions catalyzed by cytochrome P450 proteins for metabolism of steroid hormones, drugs and xenobiotics. The encoded protein has a flavin adenine dinucleotide (FAD)-binding domain and a flavodoxin-like domain which bind two cofactors, FAD and FMN, that allow it to donate electrons directly from NADPH to all microsomal P450 enzymes. Mutations in this gene cause a complex set of disorders, including apparent combined P450C17 and P450C21 deficiency, amenorrhea and disordered steroidogenesis, congenital adrenal hyperplasia and Antley-Bixler syndrome, that resemble those caused by defects in steroid metabolizing enzymes such as aromatase, 21-hydroxylase, and 17 alpha-hydroxylase.
• Source: Insect cell
• Species: Human
• Tag: His-Tag
• Form: Liquid
• Bio-activity: Specific activity is > 4000 pmol/min/mg, and is defined as the amount of enzyme that catalyze the reduction of 1.0 pmole cytochrome C by NADPH per minute at pH8.0 at 25 centigrade.
• Molecular Mass: 77.9 kDa (686aa)
• Protein length: 1-680aa
• AA Sequence: MINMGDSHVDTSSTVSEAVAEEVSL FSMTDMILFSLIVGLLTYWFLFRKK KEEVPEFTKIQTLTSSVRESSFVEK MKKTGRNIIVFYGSQTGTAEEFANR LSKDAHRYGMRGMSADPEEYDLADL SSLPEIDNALVVFCMATYGEGDPTD NAQDFYDWLQETDVDLSGVKFAVFG LGNKTYEHFNAMGKYVDKRLEQLGA QRIFELGLGDDDGNLEEDFITWREQ FWLAVCEHFGVEATGEESSIRQYEL VVHTDIDAAKVYMGEMGRLKSYENQ KPPFDAKNPFLAAVTTNRKLNQGTE RHLMHLELDISDSKIRYESGDHVAV YPANDSALVNQLGKILGADLDVVMS LNNLDEESNKKHPFPCPTSYRTALT YYLDITNPPRTNVLYELAQYASEPS EQELLRKMASSSGEGKELYLSWVVE ARRHILAILQDCPSLRPPIDHLCEL LPRLQARYYSIASSSKVHPNSVHIC AVVVEYETKAGRINKGVATNWLRAK EPVGENGGRALVPMFVRKSQFRLPF KATTPVIMVGPGTGVAPFIGFIQER AWLRQQGKEVGETLLYYGCRRSDED YLYREELAQFHRDGALTQLNVAFSR EQSHKVYVQHLLKQDREHLWKLIEG GAHIYVCGDARNMARDVQNTFYDIV AELGAMEHAQAVDYIKKLMTKGRYS LDVWS
• Endotoxin: < 1 EU/μg of protein (determined by LAL method)
• Purity: > 95% by SDS-PAGE
• Applications: SDS-PAGE, Enzyme Activity
• Storage: Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25 mg/mL (determined by absorbance at 280nm)
• Storage Buffer: Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Gene Information

• Gene Name: POR cytochrome p450 oxidoreductase [ Homo sapiens (human) ]
• Official Symbol: POR
• Synonyms: POR; cytochrome p450 oxidoreductase; CPR; CYPOR; P450R; NADPH--cytochrome P450 reductase; NADPH--hemoprotein reductase; NADPH-dependent cytochrome P450 reductase; P450 (cytochrome) oxidoreductase; EC 1.6.2.4
• Gene ID: 5447
• mRNA Refseq: NM_001395413
• Protein Refseq: NP_001382342
• MIM: 124015
• UniProt ID: P16435