Active Recombinant Human PRKCH

• Cat.No.: PRKCH-8457H
• Product Overview: The Human PRKCH was expressed in Sf9 Cells

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: Non
• Description: PKC eta is a member of the protein kinase C (PKC) family of serine- and threonine-specific protein kinases that can phosphorylate a wide variety of protein targets known to be involved in diverse cellular signaling pathways. PKC η is predominantly expressed in squamous cell epithelia and induces terminal differentiation of keratinocytes. PKC η that is endogenously expressed or overexpressed is found to associate with the cyclin E/cdk2/p21 complex in keratinocytes of mice and humans.
• Form: Recombinant protein in storage buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol).
• Bio-activity: 86 nmol phosphate incorporated into peptide substrate (ERMRPRKRQGSVRRRV) per minute per mg protein at 30°C for 15 minutes using a final concentration of 50 μM ATP (0.83 μCi/assay).
• Molecular Mass: 103.0 kDa
• Purity: ≥90% by SDS - PAGE
• Storage: Store product frozen at or below -70℃. Stable for 1 year at -70℃ as undiluted stock. Aliquot to avoidrepeated thawing and freezing.
• Shipping: dry ice

Gene Information

• Gene Name: PRKCH protein kinase C, eta [ Homo sapiens ]
• Official Symbol: PRKCH?
• Synonyms: PRKCH; protein kinase C, eta; PRKCL; protein kinase C eta type; PKC L; PKCL; PKC-L; nPKC-eta; MGC5363; MGC26269
• Gene ID: 5583
• mRNA Refseq: NM_006255
• Protein Refseq: NP_006246
• MIM: 605437
• UniProt ID: P24723
• Chromosome Location: 14q23.1
• Pathway: Calcium Regulation in the Cardiac Cell, organism-specific biosystem; Effects of PIP2 hydrolysis, organism-specific biosystem; Endothelins, organism-specific biosystem; G Protein Signaling Pathways, organism-specific biosystem; G alpha (q) signalling events, organism-specific biosystem; G alpha (z) signalling events, organism-specific biosystem; GPCR downstream signaling, organism-specific biosystem
• Function: ATP binding; enzyme binding; metal ion binding; nucleotide binding; protein kinase C activity