Active Recombinant Human Procathepsin K Protein

• Cat.No.: CTSK-22H
• Product Overview: Recombinant Human CTSK was expressed in Insect cells.

Specification

• Description: Cathepsin K, a member of the papain family of cysteine proteases, acts upon proteins such as collagen, AL amyloid, kinin and elastin. It is a lysosomal protease expressed primarily in osteoclasts, but also in other cell types such as macrophages. It functions in bone remodeling and is implicated in disease states such as atherosclerosis, arthritis and pycnodysostosis.
• Source: Insect cells
• Species: Human
• Form: Liquid. In 27.8µl 20mM sodium phosphate, pH 6.9, 500mM NaCl, 1 mM EDTA.
• Bio-activity: To activate proenzyme, add 32.5mM NaOAc, pH 3.5, to 20% of procathepsin K volume (e.g., 5µl procathepsin K + 1µl 32.5mM NaOAc, pH 3.5), and incubate at room temperature for 25-30 minutes, or at +4 centigrade for approximately seven hours.
• Molecular Mass: 35.3kDa
• Purity: ≥95% (SDS-PAGE)
• Applications: Useful tool to study enzyme kinetics, cleave target substrates and screen for inhibitors.
• Notes: Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80 centigrade.
• Stability: Use/Stability: -80 centigrade. After initial defrost, aliquot product into individual tubes and refreeze at -80 centigrade. Avoid repeated freeze/defrost cycles and extended periods unfrozen. This enzyme is stable when stored as received under the above conditions. Procedures such as dilution of the enzme followed by refreezing could lead to loss of activity.
• Storage: Long Term Storage: -80 centigrade
• Tag: Non

Gene Information

• Gene Name: CTSK cathepsin K [ Homo sapiens ]
• Official Symbol: CTSK
• Synonyms: CTSK; cathepsin K; cathepsin K (pycnodysostosis) , CTSO, CTSO2, PYCD; PKND; cathepsin O; cathepsin X; cathepsin O1; cathepsin O2; CTSO; PYCD; CTS02; CTSO1; CTSO2; MGC23107
• Gene ID: 1513
• mRNA Refseq: NM_000396
• Protein Refseq: NP_000387
• MIM: 601105
• UniProt ID: P43235
• Chromosome Location: 1q21
• Pathway: Immune System, organism-specific biosystem; Innate Immune System, organism-specific biosystem; Lysosome, organism-specific biosystem; Lysosome, conserved biosystem; Osteoclast Signaling, organism-specific biosystem; Osteoclast differentiation, organism-specific biosystem; Osteoclast differentiation, conserved biosystem
• Function: cysteine-type endopeptidase activity; cysteine-type peptidase activity; peptidase activity

Reviews

09/13/2019

This guidance can help researchers design and execute their experiments more effectively, ensuring reliable and accurate results.

01/27/2019

They have a deep understanding of the CTSK protein and can provide valuable insights into its characteristics, properties, and optimal experimental conditions.

12/14/2016

the manufacturer offers outstanding technical support, serving as a valuable resource to resolve any issues that may arise during my research.

Q&As

Can CTSK be a target for the development of novel therapeutics for periodontal diseases?

Yes, CTSK inhibitors are being investigated for their potential in preventing bone loss associated with periodontal diseases.

How does CTSK contribute to the progression of atherosclerosis, and can it be a therapeutic target for cardiovascular diseases?

CTSK has been linked to vascular calcification in atherosclerosis, and inhibiting its activity may have potential as a therapeutic strategy for cardiovascular diseases.

How might CTSK inhibitors impact the treatment landscape for bone metastasis in cancer patients?

CTSK inhibitors could potentially be used to limit bone destruction in cancer patients with bone metastasis, improving their quality of life.

Are there any other medical conditions where CTSK inhibitors could be beneficial?

CTSK inhibitors are being explored for potential therapeutic applications in conditions like rheumatoid arthritis and certain cancers due to their involvement in tissue remodeling.

Are there diagnostic applications for CTSK in identifying bone-related disorders?

While CTSK is not currently used as a diagnostic marker, its levels and activity could potentially be explored for diagnostic purposes in the future.